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J Sep Sci

Title:		Determination of volatile and non-volatile products of milk fermentation processes using capillary zone electrophoresis and solid phase microextraction coupled to gas chromatography
Author(s):		Ligor M; Jarmalaviciene R; Szumski M; Maruska A; Buszewski B;
Address:		"Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland"
Journal Title:		J Sep Sci
Year:		2008
Volume:		31
Issue:		14
Page Number:		2707 - 2713
DOI:		10.1002/jssc.200800183
ISSN/ISBN:		1615-9314 (Electronic) 1615-9306 (Linking)
Abstract:		"The aim of the investigations was to develop analytical methods for the determination of selected volatile and non-volatile organic compounds numbering among the final products of milk fermentation. The analyzed compounds were as follows: biacetyl and carboxylic acids (formic, acetic, citric, and lactic). The model yogurt was prepared under controlled conditions in our laboratory by addition of the selected bacteria (Lactobacillus bulgaricus and Streptococcus thermophilus) to the milk sample. The temperature, time, and stirring were controlled during the fermentation process. Factors considered in SPMPE-GC-FID method development included fiber exposure time, salt addition, temperature of extraction, and temperature of desorption. Various SPME fibers, for example with PDMS, CAR/PDMS, PA, and PDMS/DVB coatings, were tested to obtain the highest recovery of the investigated compounds extracted from yogurt samples. Based on these preliminary experiments, qualitative and quantitative analyses for the determination of biacetyl were performed by SPME-GC-FID. Moreover, a capillary zone electrophoresis method was developed for the determination of carboxylic acids in the yogurt samples. The buffer composition as well as deproteinization by acetonitrile were found to have a crucial effect on the analysis"
Keywords:		"Animals Chromatography, Gas/instrumentation/methods Electrophoresis, Capillary/instrumentation/methods Fatty Acids, Volatile/*analysis/metabolism *Fermentation Lactates/*analysis/metabolism Milk/chemistry/*microbiology Organic Chemicals/*analysis/metaboli;"
Notes:		"MedlineLigor, Magdalena Jarmalaviciene, Reda Szumski, Michal Maruska, Audrius Buszewski, Boguslaw eng Germany 2008/07/12 J Sep Sci. 2008 Aug; 31(14):2707-13. doi: 10.1002/jssc.200800183"