« Previous AbstractDoping-assisted low-pressure photoionization mass spectrometry for the real-time detection of lung cancer-related volatile organic compounds    Next Abstract"Identification, Expression Patterns, and Functional Characterization of Chemosensory Proteins in Dendroctonus armandi (Coleoptera: Curculionidae: Scolytinae)" »

Int J Mol Sci

Title:		Floral Scent Emission from Nectaries in the Adaxial Side of the Innermost and Middle Petals in Chimonanthus praecox
Author(s):		Li Z; Jiang Y; Liu D; Ma J; Li J; Li M; Sui S;
Address:		"College of Horticulture and Landscape Achitecture, Southwest University, Chongqing 400715, China. znli@swu.edul.cn. Key Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education, Chongqing 400715, China. znli@swu.edul.cn. Chongqing Engineering Research Center for Floriculture, Chongqing 400715, China. znli@swu.edul.cn. College of Horticulture and Landscape Achitecture, Southwest University, Chongqing 400715, China. sherneole@163.com. Key Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education, Chongqing 400715, China. sherneole@163.com. Chongqing Engineering Research Center for Floriculture, Chongqing 400715, China. sherneole@163.com. College of Horticulture and Landscape Achitecture, Southwest University, Chongqing 400715, China. liu19830222@163.com. Key Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education, Chongqing 400715, China. liu19830222@163.com. Chongqing Engineering Research Center for Floriculture, Chongqing 400715, China. liu19830222@163.com. College of Horticulture and Landscape Achitecture, Southwest University, Chongqing 400715, China. majing427@swu.edu.cn. Key Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education, Chongqing 400715, China. majing427@swu.edu.cn. Chongqing Engineering Research Center for Floriculture, Chongqing 400715, China. majing427@swu.edu.cn. College of Horticulture and Landscape Achitecture, Southwest University, Chongqing 400715, China. acejing@126.com. Key Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education, Chongqing 400715, China. acejing@126.com. Chongqing Engineering Research Center for Floriculture, Chongqing 400715, China. acejing@126.com. College of Horticulture and Landscape Achitecture, Southwest University, Chongqing 400715, China. limy@swu.edu.cn. Key Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education, Chongqing 400715, China. limy@swu.edu.cn. Chongqing Engineering Research Center for Floriculture, Chongqing 400715, China. limy@swu.edu.cn. College of Horticulture and Landscape Achitecture, Southwest University, Chongqing 400715, China. sszcq@swu.edu.cn. Key Laboratory of Horticulture Science for Southern Mountains Regions, Ministry of Education, Chongqing 400715, China. sszcq@swu.edu.cn. Chongqing Engineering Research Center for Floriculture, Chongqing 400715, China. sszcq@swu.edu.cn"
Journal Title:		Int J Mol Sci
Year:		2018
Volume:		20181022
Issue:		10
Page Number:		-
DOI:		10.3390/ijms19103278
ISSN/ISBN:		1422-0067 (Electronic) 1422-0067 (Linking)
Abstract:		"Wintersweet (Chimonanthus praecox) is a well-known traditional fragrant plant and a winter-flowering deciduous shrub that originated in China. The five different developmental stages of wintersweet, namely, flower-bud period (FB), displayed petal stage (DP), open flower stage (OF), later blooming period (LB), and wilting period (WP) were studied using a scanning electron microscope (SEM) to determine the distribution characteristics of aroma-emitting nectaries. Results showed that the floral scent was probably emitted from nectaries distributed on the adaxial side of the innermost and middle petals, but almost none on the abaxial side. The nectaries in different developmental periods on the petals differ in numbers, sizes, and characteristics. Although the distribution of nectaries on different rounds of petals showed a diverse pattern at the same developmental periods, that of the nectaries on the same round of petals showed some of regularity. The nectary is concentrated on the adaxial side of the petals, especially in the region near the axis of the lower part of the petals. Based on transcriptional sequence and phylogenetic analysis, we report one nectary development related gene CpCRC (CRABS CLAW), and the other four YABBY family genes, CpFIL (FILAMENTOUS FLOWER), CpYABBY2, CpYABBY5-1, and CpYABBY5-2 in C. praecox (accession no. MH718960-MH718964). Quantitative RT-PCR (qRT-PCR) results showed that the expression characteristics of these YABBY family genes were similar to those of 11 floral scent genes, namely, CpSAMT, CpDMAPP, CpIPP, CpGPPS1, CpGPPS2, CpGPP, CpLIS, CpMYR1, CpFPPS, CpTER3, and CpTER5. The expression levels of these genes were generally higher in the lower part of the petals than in the upper halves in different rounds of petals, the highest being in the innermost petals, but the lowest in the outer petals. Relative expression level of CpFIL, CpCRC, CpYABBY5-1, and CpLIS in the innermost and middle petals in OF stages is significant higher than that of in outer petals, respectively. SEM and qRT-PCR results in C. praecox showed that floral scent emission is related to the distribution of nectaries"
Keywords:		"Calycanthaceae/*chemistry Flowers/*chemistry Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Plant Nectar/*chemistry Plant Proteins/chemistry Chimonanthus praecox floral scent gene expression nectary;"
Notes:		"MedlineLi, Zhineng Jiang, Yingjie Liu, Daofeng Ma, Jing Li, Jing Li, Mingyang Sui, Shunzhao eng XDJK2017B031/Fundamental Research Funds for the Central Universities/ 31500573/National Natural Science Foundation of China/ cstc2016shmszx80112/Chongqing science and technology committee projects/ yjg20163042/Chongqing education committee projects/ Switzerland 2018/10/27 Int J Mol Sci. 2018 Oct 22; 19(10):3278. doi: 10.3390/ijms19103278"