| Title: | Comparison of fungal communities and nonvolatile flavor components in black Huangjiu formed using different inoculation fermentation methods |
| Author(s): | Li P; Su R; Wang Q; Liu K; Yang H; Du W; Li Z; Chen S; Xu B; Yang W; |
| Address: | "Sericulture and Apiculture Research Institute, Yunnan Academy of Agricultural Sciences, Mengzi, China. College of Wuliangye Technology and Food Engineering, Yibin Vocational and Technical College, Yibin, China. Luzhou Greenland Wine Co., Ltd., Luzhou, China" |
| DOI: | 10.3389/fmicb.2022.955825 |
| ISSN/ISBN: | 1664-302X (Print) 1664-302X (Electronic) 1664-302X (Linking) |
| Abstract: | "Black Huangjiu (BH) is a traditional alcoholic beverage in China, which is very popular among people. The different methods (simultaneous inoculation, sequential inoculation), were applied to ferment BH in this study, which were investigated the changes in the composition of fungal communities and non-volatile flavor compounds (nVFCs) by high-throughput sequencing (HTS) and ultraperformance liquid chromatography-tandem mass spectrometer (UPLC MS/MS). The results showed that Rhizopus and Saccharomyces were the predominant fungal genera throughout fermentation, and 471 nVFCs were detected in BH after fermentation. Compared to that observed simultaneous inoculation, Rhizopus increased at the end of sequential fermentation, and the contents of the organic acids and their derivatives increased significantly [variable importance in the projection (VIP) > 1.0, p < 0.05, fold change (FC) > 2], while that of lipids and lipid-like molecules decreased significantly (VIP > 1.0, p < 0.05, FC < 0.5). Through the correlation analysis of 32 nVFCs with significant differences (VIP > 1.0, p < 0.05, FC >32 or < 0.03) and the community, it was found that lipids and lipid-like molecules (12) and organic acids and their derivatives (10) were significantly (p < 0.05) negatively correlated with Saccharomyces, but they were significantly (p < 0.05) positively correlated with Rhizopus. Compared with simultaneous inoculation, BH fermented by sequential inoculation, the taste was stronger, sweeter, mellow, and softer. Our findings provide information on nVFC dynamics and will aid in the selection of beneficial strains to improve BH quality" |
| Keywords: | black Huangjiu correlation analysis fungal communities non-volatile components sequential inoculation; |
| Notes: | "PubMed-not-MEDLINELi, Pingping Su, Rui Wang, Qi Liu, Kunyi Yang, Hai Du, Wei Li, Zhengang Chen, Song Xu, Bin Yang, Wen eng Switzerland 2022/08/09 Front Microbiol. 2022 Jul 22; 13:955825. doi: 10.3389/fmicb.2022.955825. eCollection 2022" |
