| Title: | Method for rapidly discovering active components in Yupingfeng granules by UPLC-ESI-Q-TOF-MS |
| Author(s): | Li M; Yue X; Gao Y; Zhang B; Yuan C; Wu T; |
| Address: | "School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China. State Key Laboratory of New Drug and Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai, China. Sinopharm Group Guangdong Medi-World Pharmaceutical Co., Ltd., Foshan, China" |
| ISSN/ISBN: | 1096-9888 (Electronic) 1076-5174 (Linking) |
| Abstract: | "Yupingfeng granules (YPFG) were isolated from a traditional Chinese medicine (TCM) formulation composed of three herbs (Astragali Radix, Atractylodis Macrocephalae Rhizoma, and Saposhnikoviae Radix). This formulation is used in TCM to tonify qi, and it can help strengthen exterior and reduce sweating. Nevertheless, the active components of YPFG remain unclear. In this study, the chemical constituents of YPFG were systematically characterized by ultra-performance liquid chromatography coupled with electrospray ionization/ quadrupole time-of-flight mass spectrometry (UPLC-ESI-Q-TOF-MS). Fifty-eight compounds, namely, 20 flavonoids, 19 saponins, nine organic acids, four volatile coumarins, three lactones, one alkaloid, and two other components, were identified. In addition, the constituents of YPFG with the potential for in vivo bioactivities following oral administration were investigated in Sprague-Dawley rats. Thirteen compounds, namely, 11 flavonoid-related and 2 saponin-related components, were detected in rat plasma. After enriching flavonoids and saponins in YPFG by extraction, the extracts and YPFG were administrated to immunosuppressed rats, respectively. Plasma samples were analyzed by UPLC-ESI-Q-TOF-MS, and principal component analysis (PCA) confirmed that the extracts had similar effects to YPFG. This method could discover active ingredients in YPFG quickly and provide a scientific basis for quality control and mechanism research" |
| Keywords: | "Animals Chromatography, High Pressure Liquid/methods Drugs, Chinese Herbal/administration & dosage/*chemistry Flavonoids/analysis/blood Male Quality Control Rats, Sprague-Dawley Saponins/analysis/blood Spectrometry, Mass, Electrospray Ionization/*methods;" |
| Notes: | "MedlineLi, Moying Yue, Xinyi Gao, Yongjian Zhang, Bei Yuan, Chunping Wu, Tong eng 16XD1423500/Program of Shanghai Technology Research Leader/ ZYBZH-C-GD-08/National Traditional Chinese Medicine Standardization Project/ England 2020/08/14 J Mass Spectrom. 2020 Oct; 55(10):e4627. doi: 10.1002/jms.4627" |
