| Title: | High-performance liquid chromatography quantification of enantiomers of a Dihydroxylated tetrahydrofuran natural product |
| Author(s): | Li K; Fissette SD; Buchinger TJ; Middleton ZE; Boyer A; Li W; |
| Address: | "Department of Fisheries and Wildlife, Room 13 Natural Resources Building, Michigan State University, East Lansing, MI, USA. School of Chemistry, Joseph Black Building, University of Glasgow, Glasgow, UK" |
| ISSN/ISBN: | 1520-636X (Electronic) 0899-0042 (Linking) |
| Abstract: | "Both enantiomers of petromyroxol are putative pheromones in sea lamprey (Petromyzon marinus). Here, we describe the separation and quantification of the petromyroxol enantiomers using high-performance liquid chromatography tandem mass spectrometry. The separation was tested on a wide range of chiral columns with normal phases, and effects of the chromatographic parameters such as mobile phase and temperature on the separation were optimized. The AD-H column showed the best separation of enantiomers with n-hexane and ethanol as the mobile phase. The enantiomers were detected by multiple reaction monitoring with a positive atmospheric-pressure chemical ionization on triple quadrupole mass spectrometer. Validation revealed that the method was specific, accurate, and precise. The validated method was applied to measure the amount of petromyroxol enantiomers in water conditioned with sea lamprey larvae, the source of the putative pheromone. This method will be applied in quantifying the natural scalemic petromyroxol mixture, enabling further investigations of a rare non-racemic enantiomeric pheromone mixture in a vertebrate species" |
| Keywords: | Hplc-ms/ms atmospheric-pressure chemical ionization fish pheromone quantitative analysis sea lamprey; |
| Notes: | "PublisherLi, Ke Fissette, Skye D Buchinger, Tyler J Middleton, Zoe E Boyer, Alistair Li, Weiming eng 2018/05/24 Chirality. 2018 May 23. doi: 10.1002/chir.22978" |
