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J Chromatogr A

Title:		Artifacts in trimethylsilyl derivatization reactions and ways to avoid them
Author(s):		Little JL;
Address:		"Eastman Chemical Company, Kingsport, TN 37662-5150, USA. jllittle@eastman.com"
Journal Title:		J Chromatogr A
Year:		1999
Volume:		844
Issue:		1-Feb
Page Number:		1 - 22
DOI:		10.1016/s0021-9673(99)00267-8
ISSN/ISBN:		0021-9673 (Print) 0021-9673 (Linking)
Abstract:		"Trimethylsilyl derivatives are routinely employed in gas chromatography to increase the volatility and stability of organic compounds containing active hydrogens. Normally only the desired derivative is formed when organic compounds are derivatized with common silylation reagents. However, some compounds form additional unexpected derivatives or by-products (artifacts). Artifact formation leads to multiple peaks for the same compound or unexpected components in the gas chromatographic analysis of mixtures. This review includes silylation artifacts identified in our laboratory by mass spectrometry during the last 20 years and references to those found in the literature. Also, means of avoiding artifact formation are discussed in detail"
Keywords:		"Aldehydes/analysis Amides/analysis Carboxylic Acids/analysis *Chromatography, Gas/methods False Positive Reactions Ketones/analysis Mass Spectrometry Solvents *Trimethylsilyl Compounds;"
Notes:		"MedlineLittle, J L eng Review Netherlands 1999/07/10 J Chromatogr A. 1999 Jun 4; 844(1-2):1-22. doi: 10.1016/s0021-9673(99)00267-8"