| Title: | Comparison of volatile organic compounds between cigarette smoke condensate (CSC) and extract (CSE) samples |
| Author(s): | Kim YH; An YJ; Jo S; Lee SH; Lee SJ; Choi SJ; Lee K; |
| Address: | "Jeonbuk Department of Inhalation Research, Korea Institute of Toxicology, Jeongeup 56212, Republic of Korea. Human and Environmental Toxicology, University of Science and Technology, Daejeon 34113, Republic of Korea. Department of Toxicology Evaluation, Konyang University, Daejeon 35365 Republic of Korea. National Center for Efficacy Evaluation of Respiratory Disease Product, Korea Institute of Toxicology, Jeongeup 56212, Republic of Korea" |
| ISSN/ISBN: | 2233-6567 (Print) 2233-6567 (Electronic) 2233-6567 (Linking) |
| Abstract: | "Cigarette smoke is a major risk factor for several diseases, including chronic obstructive pulmonary and cardiovascular diseases. The toxicity of the cigarette smoke can be determined in vitro. The cytotoxicity test of the cigarette smoke is commonly conducted using the cigarette smoke condensate (CSC) and cigarette smoke extract (CSE). The CSC and CSE methods are well known for sampling of the particles and water-soluble compounds in the cigarette smoke, respectively. In this study, the CSC and CSE were analyzed by using a gas chromatography-mass spectrometry (GC-MS) system equipped with a wax column for separation of the volatile organic compounds. The cytotoxic effect of the CSC and CSE were evaluated thoroughly by comparing the analytical results of the CSC and CSE samples. The total concentration of the volatile organic compounds detected in the CSC sample was similar to that in the CSE sample based on the peak area. Except for the dimethyl sulfoxide solvent, nicotine had the highest concentration in the CSC sample, while acetonitrile had the highest concentration in the CSE sample. The compositions were as follows: (1) CSC sample: 55.8% nicotine, 18.0% nicotyrine, 3.20% 1,2,3-propanetriol, triacetate, 1.28% ethyl chloride, 1.22% phenol, etc. and (2) CSE sample: 18.7% acetonitrile, 18.0% acetone, 12.5% 2-hydroxy-2-methyl-propanenitrile, 8.98% nicotine, 5.86% nicotyrine, etc. In this manner, to accurately examine the cytotoxicity of the cigarette smoke using CSC or CSE, the components and their concentrations in the CSC and CSE samples should be considered" |
| Keywords: | cigarette smoke condensate (CSC) cigarette smoke extract (CSE) gas chromatography -mass spectrometry (GC-MS) volatile organic compound (VOC); |
| Notes: | "PubMed-not-MEDLINEKim, Yong-Hyun An, Young-Ji Jo, Seongwon Lee, Sang-Hyub Lee, Sang Jin Choi, Seong-Jin Lee, Kyuhong eng 14182MFDS977/Ministry of Food and Drug Safety/ Korea (South) 2018/10/06 Environ Health Toxicol. 2018 Sep; 33(3):e2018012-0. doi: 10.5620/eht.e2018012. Epub 2018 Sep 28" |
