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J Microbiol Biotechnol

Title:		Functional mechanism of plant growth retardation by Bacillus subtilis IJ-31 and its allelochemicals
Author(s):		Kim WC; Rhee IK;
Address:		"Department of Agricultural Chemistry, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, Korea"
Journal Title:		J Microbiol Biotechnol
Year:		2012
Volume:		22
Issue:		10
Page Number:		1375 - 1380
DOI:		10.4014/jmb.1207.07031
ISSN/ISBN:		1738-8872 (Electronic) 1017-7825 (Linking)
Abstract:		"We previously isolated a rhizobacterium (Bacillus subtilis IJ-31) and demonstrated that its associated allelochemicals could indicate plant growth retardation. However, little is known about how the growth of plants is regulated by B. subtilis IJ-31 and its allelochemicals. In this study, we investigated whether plant growth retardation in this relationship occurred through the inhibition of gibberellin (GA) biosynthesis. GA 3beta-hydroxylase activity was found to be inhibited by B. subtilis IJ-31 and hydrocinnamic acid (HCA), which is one of the allelochemicals produced by B. subtilis IJ-31. Additionally, thin layer chromatography (TLC) demonstrated that B. subtilis IJ-31 culture broth and HCA both inhibit GA 3beta-hydroxylase (MBP-GA4) activity. The retardation of plants by HCA was then confirmed in vivo and in vitro using a Ryegrass and Arabidopsis growth retardation assay. Furthermore, treatment with either B. subtilis IJ-31 culture extract or its allelochemicals resulted in significant down-regulation of XTR9 gene expression in Arabidopsis. Overall, we identified the functional mechanism of plant growth retardation by B. subtilis IJ-31 and its allelochemicals"
Keywords:		"Arabidopsis/*drug effects/enzymology/genetics/*growth & development Bacillus subtilis/*chemistry Capsicum/drug effects/enzymology/genetics/growth & development Chromatography, Thin Layer Down-Regulation Enzyme Activation Enzyme Assays Enzyme Inhibitors/ch;"
Notes:		"MedlineKim, Won-Chan Rhee, In-Koo eng Korea (South) 2012/10/19 J Microbiol Biotechnol. 2012 Oct; 22(10):1375-80. doi: 10.4014/jmb.1207.07031"