| Title: | A mechanism for the coordination of proliferation and differentiation by spatial regulation of Fus2p in budding yeast |
| Address: | "Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA" |
| ISSN/ISBN: | 1549-5477 (Electronic) 0890-9369 (Print) 0890-9369 (Linking) |
| Abstract: | "Yeast cells induce the genes required for mating prior to the completion of mitosis. To ensure proper cell cycle progression prior to mating differentiation, a key cytoplasmic regulator of cell fusion, Fus2p, is sequestered in the nucleus by cyclin-dependent kinase (Cdk). In response to pheromone signaling, the mitogen-activated protein kinase Fus3p phosphorylates Ser 84 in Fus2p to drive nuclear export. We found that Fus3p becomes active and phosphorylates S84 as early as S phase, raising the question of how Cdk prevents inappropriate activation of Fus2p. Countering Fus3p, Cdk and a p21-activated kinase, Cla4p, maintain Fus2p's nuclear localization by phosphorylating Ser 67, which drives nuclear import and inhibits nuclear export. When Cdk and Cla4p activities drop after cell division, Fus3p promotes Fus2p export both via S84 phosphorylation and by down-regulating S67 phosphorylation. Thus, potential premature activation of Fus2p in mitosis is prevented by cell cycle-dependent phosphorylation that overrides the mating pheromone-induced phosphorylation that drives nuclear export" |
| Keywords: | "Active Transport, Cell Nucleus Amino Acid Sequence *Cell Differentiation Cell Nucleus/*metabolism *Cell Proliferation Cyclin-Dependent Kinases/metabolism Cytoskeletal Proteins/genetics/*metabolism Karyopherins/metabolism Membrane Proteins/genetics/*metabo;" |
| Notes: | "MedlineKim, Junwon Rose, Mark D eng R01 GM037739/GM/NIGMS NIH HHS/ GM37739/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural 2012/05/17 Genes Dev. 2012 May 15; 26(10):1110-21. doi: 10.1101/gad.187260.112" |
