| Title: | Diversification of JAZ-MYC signaling function in immune metabolism |
| Author(s): | Johnson LYD; Major IT; Chen Y; Yang C; Vanegas-Cano LJ; Howe GA; |
| Address: | "MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI, 48824, USA. Cell and Molecular Biology Program, Michigan State University, East Lansing, MI, 48824, USA. Molecular Plant Sciences Program, Michigan State University, East Lansing, MI, 48824, USA. Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, 48824, USA. Plant Resilience Institute, Michigan State University, East Lansing, MI, 48824, USA" |
| ISSN/ISBN: | 1469-8137 (Electronic) 0028-646X (Print) 0028-646X (Linking) |
| Abstract: | "Jasmonate (JA) re-programs metabolism to confer resistance to diverse environmental threats. Jasmonate stimulates the degradation of JASMONATE ZIM-DOMAIN (JAZ) proteins that repress the activity of MYC transcription factors. In Arabidopsis thaliana, MYC and JAZ are encoded by 4 and 13 genes, respectively. The extent to which expansion of the MYC and JAZ families has contributed to functional diversification of JA responses is not well understood. Here, we investigated the role of MYC and JAZ paralogs in controlling the production of defense compounds derived from aromatic amino acids (AAAs). Analysis of loss-of-function and dominant myc mutations identified MYC3 and MYC4 as the major regulators of JA-induced tryptophan metabolism. We developed a JAZ family-based, forward genetics approach to screen randomized jaz polymutants for allelic combinations that enhance tryptophan biosynthetic capacity. We found that mutants defective in all members (JAZ1/2/5/6) of JAZ group I over-accumulate AAA-derived defense compounds, constitutively express marker genes for the JA-ethylene branch of immunity and are more resistant to necrotrophic pathogens but not insect herbivores. In defining JAZ and MYC paralogs that regulate the production of amino-acid-derived defense compounds, our results provide insight into the specificity of JA signaling in immunity" |
| Keywords: | "*Arabidopsis Proteins/metabolism Proto-Oncogene Proteins c-myc/genetics/metabolism Repressor Proteins/metabolism Tryptophan/metabolism Signal Transduction *Arabidopsis/metabolism Cyclopentanes/metabolism Oxylipins/metabolism Gene Expression Regulation, Pl;" |
| Notes: | "MedlineJohnson, Leah Y D Major, Ian T Chen, Yani Yang, Changxian Vanegas-Cano, Leidy J Howe, Gregg A eng T32 GM110523/GM/NIGMS NIH HHS/ T32-GM110523/NH/NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't England 2023/07/05 New Phytol. 2023 Sep; 239(6):2277-2291. doi: 10.1111/nph.19114. Epub 2023 Jul 4" |
