| Title: | Development of radiation indicators to distinguish between irradiated and non-irradiated herbal medicines using HPLC and GC-MS |
| Author(s): | Kim MJ; Ki HA; Kim WY; Pal S; Kim BK; Kang WS; Song JM; |
| Address: | "Research Institute of Pharmaceutical Sciences and College of Pharmacy, Seoul National University, Seoul, 151-742, South Korea" |
| DOI: | 10.1007/s00216-010-3935-x |
| ISSN/ISBN: | 1618-2650 (Electronic) 1618-2642 (Linking) |
| Abstract: | "The effects of high dose gamma-irradiation on six herbal medicines were investigated using gas chromatography-mass spectrometry (GC/MS) and high-performance liquid chromatography (HPLC). Herbal medicines were irradiated at 0-50 kGy with (60)Co irradiator. HPLC was used to quantify changes of major components including glycyrrhizin, cinnamic acid, poncirin, hesperidin, berberine, and amygdalin in licorice, cinnamon bark, poncirin immature fruit, citrus unshiu peel, coptis rhizome, and apricot kernel. No significant differences were found between gamma-irradiated and non-irradiated samples with regard to the amounts of glycyrrhizin, berberine, and amygdalin. However, the contents of cinnamic acid, poncirin, and hesperidin were increased after irradiation. Volatile compounds were analyzed by GC/MS. The relative proportion of ketone in licorice was diminished after irradiation. The relative amount of hydrocarbons in irradiated cinnamon bark and apricot kernel was higher than that in non-irradiated samples. Therefore, ketone in licorice and hydrocarbons in cinnamon bark and apricot kernel can be considered radiolytic markers. Three unsaturated hydrocarbons, i.e., 1,7,10-hexadecatriene, 6,9-heptadecadiene, and 8-heptadecene, were detected only in apricot kernels irradiated at 25 and 50 kGy. These three hydrocarbons could be used as radiolytic markers to distinguish between irradiated (>25 kGy) and non-irradiated apricot kernels" |
| Keywords: | "Chromatography, High Pressure Liquid/methods Cinnamomum zeylanicum/chemistry/radiation effects Citrus/chemistry/radiation effects Coptis/chemistry/radiation effects Flavonoids Gamma Rays *Gas Chromatography-Mass Spectrometry/methods Glycyrrhiza/chemistry/;" |
| Notes: | "MedlineKim, Min Jung Ki, Hyeon A Kim, Won Young Pal, Sukdeb Kim, Byeong Keun Kang, Woo Suk Song, Joon Myong eng Research Support, Non-U.S. Gov't Germany 2010/07/14 Anal Bioanal Chem. 2010 Sep; 398(2):943-53. doi: 10.1007/s00216-010-3935-x. Epub 2010 Jul 11" |
