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Mol Cell Biol

Title:		Binding of alpha-factor pheromone to Saccharomyces cerevisiae a cells: dissociation constant and number of binding sites
Author(s):		Jenness DD; Burkholder AC; Hartwell LH;
Address:
Journal Title:		Mol Cell Biol
Year:		1986
Volume:		6
Issue:		1
Page Number:		318 - 320
DOI:		10.1128/mcb.6.1.318-320.1986
ISSN/ISBN:		0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking)
Abstract:		"The number of alpha-factor binding sites on yeast MATa cells (8,000) and the equilibrium dissociation constant (6 X 10(-9) M) were determined from direct binding experiments. These values correct our previously reported estimates (D. D. Jennes, A. C. Burkholder, and L. H. Hartwell, Cell 35:521-529, 1983) that were based on indirect isotope dilution studies, and they lead to a revised rate constant for the association process (kon = 3 X 10(5) mol-1 s-1)"
Keywords:		"Kinetics Mating Factor Peptides/*metabolism Pheromones/metabolism Receptors, Cell Surface/*metabolism Receptors, Mating Factor *Receptors, Peptide Saccharomyces cerevisiae/*metabolism *Transcription Factors;"
Notes:		"MedlineJenness, D D Burkholder, A C Hartwell, L H eng GM17709/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1986/01/01 Mol Cell Biol. 1986 Jan; 6(1):318-20. doi: 10.1128/mcb.6.1.318-320.1986"