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Curr Microbiol

Title:		Increased pheromone cCF10 expression in Enterococcus faecalis biofilm formed by isolates from renal transplant patients
Author(s):		Jarzembowski T; Daca A; Bryl E; Wisniewska K; Golebiewska J; Debska-Slizien A; Rutkowski B; Witkowski J;
Address:		"Department of Microbiology, Medical University of Gdansk, Do Studzienki 38 Street, 80-227 Gdansk, Poland. doktj@univ.gda.pl"
Journal Title:		Curr Microbiol
Year:		2012
Volume:		20120821
Issue:		6
Page Number:		656 - 659
DOI:		10.1007/s00284-012-0202-8
ISSN/ISBN:		1432-0991 (Electronic) 0343-8651 (Linking)
Abstract:		"Renal transplant recipients are at a high risk of developing infectious complications even caused by commensal bacteria. This is because of various physiological non-immunological, and immunological protective mechanisms are not fully efficient in RTx patients. Therefore, rapid and precise diagnostic tools are essential in this particular group of patients. We aimed to develop simple and sensitive protocol Flow-Fish for the study of gene expression in enterococci and to compare expression of genes involved in virulence regulation in biofilm and planktonic form of Enterococcus faecalis. Proper optimization of the method was demonstrated with analysis of dehydrogenase gene expression. According to expectation reduction of the dehydrogenase gene expression was observed in biofilm. Furthermore, expression of studied gene was higher in clinical than in commensal strains. We have also found that in contrast to dehydrogenase gene, pheromone cCF10 gene expression increasing then clinical strains formed biofilm"
Keywords:		"Bacterial Proteins/genetics/metabolism Biofilms/*growth & development Enterococcus faecalis/isolation & purification/metabolism/*pathogenicity Feces/microbiology Flow Cytometry *Gene Expression Regulation, Bacterial Glucose 1-Dehydrogenase/genetics/metabo;"
Notes:		"MedlineJarzembowski, Tomasz Daca, Agnieszka Bryl, Ewa Wisniewska, Katarzyna Golebiewska, Justyna Debska-Slizien, Alicja Rutkowski, Boleslaw Witkowski, Jacek eng Evaluation Study Research Support, Non-U.S. Gov't 2012/08/22 Curr Microbiol. 2012 Dec; 65(6):656-9. doi: 10.1007/s00284-012-0202-8. Epub 2012 Aug 21"