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Fungal Genet Biol

Title:		Functional expression of the Candida albicans alpha-factor receptor in Saccharomyces cerevisiae
Author(s):		Janiak AM; Sargsyan H; Russo J; Naider F; Hauser M; Becker JM;
Address:		"Department of Microbiology, University of Tennessee, Knoxville, TN 37996, USA"
Journal Title:		Fungal Genet Biol
Year:		2005
Volume:		42
Issue:		4
Page Number:		328 - 338
DOI:		10.1016/j.fgb.2005.01.006
ISSN/ISBN:		1087-1845 (Print) 1087-1845 (Linking)
Abstract:		"Candida albicans genes involved in mating have been identified previously by homology to Saccharomyces cerevisiae mating pathway components. The C. albicans genome encodes CaSte2p, a homolog of the S. cerevisiae alpha-mating pheromone receptor Ste2p, and two potential pheromones, alpha-F13 (GFRLTNFGYFEPG) and alpha-F14 (GFRLTNFGYFEPGK). The response of several C. albicans strains to the synthesized peptides was determined. The alpha-F13 was degraded by a C. albicans MTLa strain but not by S. cerevisiae MATa cells. The CaSTE2 gene was cloned and expressed in a ste2-deleted strain of S. cerevisiae. Growth arrest and beta-galactosidase activity induced from a FUS1-lacZ reporter construct increased in a dose-dependent manner upon exposure of transgenic S. cerevisiae to alpha-F13. Mating between the strain expressing CaSTE2 and an opposite mating type was mediated by alpha-F13 and not by the S. cerevisiae alpha-factor. The results indicated that CaSte2p effectively coupled to the S. cerevisiae signal transduction pathway. Functional expression of CaSte2p in S. cerevisiae provides a well-defined system for studying the biochemistry and molecular biology of the C. albicans pheromone and its receptor"
Keywords:		"Amino Acid Sequence Candida albicans/genetics/growth & development/*metabolism/physiology Culture Media Fungal Proteins/genetics/metabolism *Gene Expression Regulation, Fungal Mating Factor Peptides/chemical synthesis/chemistry/metabolism Receptors, Matin;"
Notes:		"MedlineJaniak, Agnieszka M Sargsyan, Hasmik Russo, Joe Naider, Fred Hauser, Melinda Becker, Jeffrey M eng 2005/03/08 Fungal Genet Biol. 2005 Apr; 42(4):328-38. doi: 10.1016/j.fgb.2005.01.006"