| Title: | Functional characteristic analysis of three odorant-binding proteins from the sweet potato weevil (Cylas formicarius) in the perception of sex pheromones and host plant volatiles |
| Author(s): | Hua J; Pan C; Huang Y; Li Y; Li H; Wu C; Chen T; Ma D; Li Z; |
| Address: | "Institute of Integrative Plant Biology, Jiangsu Key Laboratory of Phylogenomics & Comparative Genomics, School of Life Sciences, Jiangsu Normal University, Jiangsu, China. Sweet Potato Laboratory, Maize Research Institute, Guangxi Academy of Agricultural Sciences, Guangxi, China. Xuzhou Academy of Agricultural Sciences/Sweet Potato Research Institute, CAAS, Jiangsu, China" |
| ISSN/ISBN: | 1526-4998 (Electronic) 1526-498X (Linking) |
| Abstract: | "BACKGROUND: The sweet potato weevil, Cylas formicarius, is the most serious pest of sweet potato worldwide. The molecular mechanism of sex pheromone recognition in C. formicarius has not been reported. Odorant-binding proteins (OBPs) play a critical role in selectively binding and transporting pheromones or other odors to the surface of olfactory receptor neurons through the aqueous sensillar lymph, therefore the function of sweet potato OBPs is worth studying. RESULTS: Herein, the CforOBP1-3 genes encoding three classical OBPs were cloned in C. formicarius by reverse transcription-polymerase chain reaction. Phylogenetic analysis showed that CforOBP1-3 were homologous genes, but the relationship between CforOBP2 and CforOBP3 was closest among the three genes. In addition, real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays demonstrated that the expression of CforOBP1 was higher in the antennae and legs of female and male insects, while CforOBP2 and CforOBP3 were mainly expressed in the antennae of male insects. The fluorescent competitive binding assay results indicated that CforOBP1-3 had strong binding affinities to sex pheromones and other tested ligands. Finally, the mRNA expression of CforOBP1-3 was successfully inhibited by RNA interference, and in vivo behavioral experiments showed that CforOBP1-3-deficient C. formicarius was partly anosmic and lost some of its ability to locate sex pheromones and host plant volatiles. CONCLUSION: These results suggested that CforOBP1 was shown to be involved in the process of weevils feeding and finding sweet potato, and CforOBP2-3 were mainly involved in the mating behavior of adult male weevils" |
| Keywords: | "Animals Carrier Proteins Female Insect Proteins/genetics/metabolism *Ipomoea batatas Male Odorants Perception Pheromones Phylogeny *Receptors, Odorant/genetics/metabolism *Sex Attractants *Weevils/metabolism Cylas formicarius RNA interference behavior res;" |
| Notes: | "MedlineHua, Jinfeng Pan, Chao Huang, Yongmei Li, Yanqing Li, Huifeng Wu, Cuirong Chen, Tianyuan Ma, Daifu Li, Zongyun eng Guinongke2017JZ05/Science and Technology Development Foundation of Guangxi academy of agricultural sciences/ Guinongke2018YM18/Science and Technology Development Foundation of Guangxi academy of agricultural sciences/ CARS-10-B3/Earmarked Fund for China Agriculture Research System/ CARS-10-C19/Earmarked Fund for China Agriculture Research System/ 2016JJB130253/Natural Science Foundation of Guangxi Province of China/ KYCX18-2126/Postgraduate Research&Practice innovation program of Jiangsu Province of China/ Priority Academic Program Development of Jiangsu Higher Education Institutions/ Jiangsu Province/ Natural Science Foundation of Guangxi Province/ 31660627/National Natural Science Foundation of China/ England 2020/07/23 Pest Manag Sci. 2021 Jan; 77(1):300-312. doi: 10.1002/ps.6019. Epub 2020 Aug 16" |
