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Metabolomics

Title:		A comparison of sample preparation methods for extracting volatile organic compounds (VOCs) from equine faeces using HS-SPME
Author(s):		Hough R; Archer D; Probert C;
Address:		"Department of Cellular and Molecular Physiology, University of Liverpool, Liverpool, UK. rachael.hough@liverpool.ac.uk. Department of Epidemiology and Population Health, University of Liverpool, Liverpool, UK. Department of Cellular and Molecular Physiology, University of Liverpool, Liverpool, UK"
Journal Title:		Metabolomics
Year:		2018
Volume:		20180104
Issue:		2
Page Number:		19 -
DOI:		10.1007/s11306-017-1315-7
ISSN/ISBN:		1573-3882 (Print) 1573-3890 (Electronic) 1573-3882 (Linking)
Abstract:		"INTRODUCTION: Disturbance to the hindgut microbiota can be detrimental to equine health. Metabolomics provides a robust approach to studying the functional aspect of hindgut microorganisms. Sample preparation is an important step towards achieving optimal results in the later stages of analysis. The preparation of samples is unique depending on the technique employed and the sample matrix to be analysed. Gas chromatography mass spectrometry (GCMS) is one of the most widely used platforms for the study of metabolomics and until now an optimised method has not been developed for equine faeces. OBJECTIVES: To compare a sample preparation method for extracting volatile organic compounds (VOCs) from equine faeces. METHODS: Volatile organic compounds were determined by headspace solid phase microextraction gas chromatography mass spectrometry (HS-SPME-GCMS). Factors investigated were the mass of equine faeces, type of SPME fibre coating, vial volume and storage conditions. RESULTS: The resultant method was unique to those developed for other species. Aliquots of 1000 or 2000 mg in 10 ml or 20 ml SPME headspace were optimal. From those tested, the extraction of VOCs should ideally be performed using a divinylbenzene-carboxen-polydimethysiloxane (DVB-CAR-PDMS) SPME fibre. Storage of faeces for up to 12 months at - 80 degrees C shared a greater percentage of VOCs with a fresh sample than the equivalent stored at - 20 degrees C. CONCLUSIONS: An optimised method for extracting VOCs from equine faeces using HS-SPME-GCMS has been developed and will act as a standard to enable comparisons between studies. This work has also highlighted storage conditions as an important factor to consider in experimental design for faecal metabolomics studies"
Keywords:		Equine Faeces Gc-ms Spme Volatile organic compounds;
Notes:		"PubMed-not-MEDLINEHough, Rachael Archer, Debra Probert, Christopher eng 2018/01/26 Metabolomics. 2018; 14(2):19. doi: 10.1007/s11306-017-1315-7. Epub 2018 Jan 4"