| Title: | Detection of signature volatiles for cariogenic microorganisms |
| Author(s): | Hertel M; Preissner R; Gillissen B; Schmidt-Westhausen AM; Paris S; Preissner S; |
| Address: | "Department of Oral Medicine, Dental Radiology and Oral Surgery, Charite - Universitatsmedizin Berlin, Berlin, Germany. Structural Bioinformatics Group, Institute for Physiology, Charite - Universitatsmedizin Berlin, Berlin, Germany. German Cancer Consortium (DKTK), Heidelberg, Germany. Hematology, Oncology and Tumor Immunology, Charite - Universitatsmedizin Berlin, Berlin, Germany. Department of Operative and Preventive Dentistry, Charite - Universitatsmedizin Berlin, Assmannshauser Str. 4-6, 14197, Berlin, Germany. Department of Operative and Preventive Dentistry, Charite - Universitatsmedizin Berlin, Assmannshauser Str. 4-6, 14197, Berlin, Germany. saskia.preissner@charite.de" |
| Journal Title: | Eur J Clin Microbiol Infect Dis |
| DOI: | 10.1007/s10096-015-2536-1 |
| ISSN/ISBN: | 1435-4373 (Electronic) 0934-9723 (Linking) |
| Abstract: | "The development of a breath test by the identification of volatile organic compounds (VOCs) emitted by cariogenic bacteria is a promising approach for caries risk assessment and early caries detection. The aim of the present study was to investigate the volatile profiles of three major cariogenic bacteria and to assess whether the obtained signatures were species-specific. Therefore, the headspaces above cultures of Streptococcus mutans, Lactobacillus salivarius and Propionibacterium acidifaciens were analysed after 24 and 48 h of cultivation using gas chromatography and mass spectrometry. A volatile database was queried for the obtained VOC profiles. Sixty-four compounds were detected within the analysed culture headspaces and were absent (36) or at least only present in minor amounts (28) in the control headspace. For S. mutans 18, for L. salivarius three and for P. acidifaciens five compounds were found to be unique signature VOCs. Database matching revealed that the identified signatures of all bacteria were unique. Furthermore, 13 of the 64 detected substances have not been previously reported to be emitted by bacteria or fungi. Specific VOC signatures were found in all the investigated bacteria cultures. The obtained results encourage further research to investigate the transferability to in vivo conditions towards the development of a breath test" |
| Keywords: | Breath Tests/*methods Dental Caries/*diagnosis/microbiology Gas Chromatography-Mass Spectrometry Humans Lactobacillus/*metabolism Propionibacterium/*metabolism Risk Assessment Streptococcus mutans/*metabolism Volatile Organic Compounds/*analysis; |
| Notes: | "MedlineHertel, M Preissner, R Gillissen, B Schmidt-Westhausen, A M Paris, S Preissner, S eng Germany 2015/11/28 Eur J Clin Microbiol Infect Dis. 2016 Feb; 35(2):235-44. doi: 10.1007/s10096-015-2536-1. Epub 2015 Nov 26" |
