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Elife

Title:		"Circadian oscillations in Trichoderma atroviride and the role of core clock components in secondary metabolism, development, and mycoparasitism against the phytopathogen Botrytis cinerea"
Author(s):		Henriquez-Urrutia M; Spanner R; Olivares-Yanez C; Seguel-Avello A; Perez-Lara R; Guillen-Alonso H; Winkler R; Herrera-Estrella A; Canessa P; Larrondo LF;
Address:		"ANID - Millennium Science Initiative Program - Millennium Institute for Integrative Biology (iBio), Santiago, Chile. Pontificia Universidad Catolica de Chile, Biological Sciences Faculty, Molecular Genetics and Microbiology Department, Santiago, Chile. Centro de Biotecnologia Vegetal, Facultad de Ciencias de la Vida, Universidad Andres, Santiago, Chile. Department of Biotechnology and Biochemistry, Cinvestav Unidad Irapuato, Irapuato, Mexico. Laboratorio de expresion genica y desarrollo en hongos, Unidad de Genomica Avanzada-LANGEBIO, Irapuato, Mexico"
Journal Title:		Elife
Year:		2022
Volume:		20220811
Issue:
Page Number:		-
DOI:		10.7554/eLife.71358
ISSN/ISBN:		2050-084X (Electronic) 2050-084X (Linking)
Abstract:		"Circadian clocks are important for an individual's fitness, and recent studies have underlined their role in the outcome of biological interactions. However, the relevance of circadian clocks in fungal-fungal interactions remains largely unexplored. We sought to characterize a functional clock in the biocontrol agent Trichoderma atroviride to assess its importance in the mycoparasitic interaction against the phytopathogen Botrytis cinerea. Thus, we confirmed the existence of circadian rhythms in T. atroviride, which are temperature-compensated and modulated by environmental cues such as light and temperature. Nevertheless, the presence of such molecular rhythms appears to be highly dependent on the nutritional composition of the media. Complementation of a clock null (Deltafrq) Neurospora crassa strain with the T. atroviride-negative clock component (tafrq) restored core clock function, with the same period observed in the latter fungus, confirming the role of tafrq as a bona fide core clock component. Confrontation assays between wild-type and clock mutant strains of T. atroviride and B. cinerea, in constant light or darkness, revealed an inhibitory effect of light on T. atroviride's mycoparasitic capabilities. Interestingly, when confrontation assays were performed under light/dark cycles, T. atroviride's overgrowth capacity was enhanced when inoculations were at dawn compared to dusk. Deleting the core clock-negative element FRQ in B. cinerea, but not in T. atroviride, was vital for the daily differential phenotype, suggesting that the B. cinerea clock has a more significant influence on the result of this interaction. Additionally, we observed that T. atroviride clock components largely modulate development and secondary metabolism in this fungus, including the rhythmic production of distinct volatile organic compounds (VOCs). Thus, this study provides evidence on how clock components impact diverse aspects of T. atroviride lifestyle and how daily changes modulate fungal interactions and dynamics"
Keywords:		*Botrytis/growth & development/metabolism/radiation effects *CLOCK Proteins/metabolism *Circadian Rhythm/radiation effects *Fungal Proteins/metabolism *Hypocreales/growth & development/metabolism/radiation effects Light *Microbial Interactions *Secondary;
Notes:		"MedlineHenriquez-Urrutia, Marlene Spanner, Rebecca Olivares-Yanez, Consuelo Seguel-Avello, Aldo Perez-Lara, Rodrigo Guillen-Alonso, Hector Winkler, Robert Herrera-Estrella, Alfredo Canessa, Paulo Larrondo, Luis F eng HHMI/Howard Hughes Medical Institute/ England 2022/08/12 Elife. 2022 Aug 11; 11:e71358. doi: 10.7554/eLife.71358"