| Title: | An antenna-biased carboxylesterase is specifically active to plant volatiles in Spodoptera exigua |
| Author(s): | He P; Zhang YN; Yang K; Li ZQ; Dong SL; |
| Address: | "Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Guizhou University, Ministry of Education, Guiyang 550025, China. Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; College of Life Sciences, Huaibei Normal University, Huaibei 235000, China. Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China. Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: sldong@njau.edu.cn" |
| DOI: | 10.1016/j.pestbp.2015.03.009 |
| ISSN/ISBN: | 1095-9939 (Electronic) 0048-3575 (Linking) |
| Abstract: | "Odorant-degrading enzymes (ODEs) in sensillar lymph are proposed to play important roles in the maintenance of the sensitivity of the olfactory sensilla, by timely degrading the odorants that have already fulfilled the activation of the odorant receptor (OR). Here we reported the cloning and characterization of an ODE gene (SexiCXE10) from the polyphagous insect pest Spodoptera exigua. SexiCXE10 is a carboxylesterase (CXE) gene, encoding a protein with 538 amino acid residues, and bearing typical characteristics of Carboxyl/cholinesterase (CCE, EC 3.1.1.1.) gene family. Tissue-temporal expression pattern by qPCR revealed that the SexiCXE10 mRNA was highly antenna biased, and maintained at high level throughout the adult stage. Further fluorescence in situ hybridization demonstrated that SexiCXE10 mRNA signal was detected under sensilla basiconica and short and long sensilla trichodea. Finally, enzymatic study using purified recombinant enzyme showed that SexiCXE10 had high activity specifically for ester plant volatiles with 7-10 carbon atoms, while no activity was found with S. exigua sex pheromone components and plant volatiles with more carbon atoms. In addition, SexiCXE10 displayed lower activity at acidic pH (pH 5.0), while higher activity was found at neutral and alkaline conditions (pH 6.5-9.0). Our results suggest that SexiCXE10 may play an important role in the degradation of the host plant volatiles, and thus contributes to the high sensitivity of the olfactory system in S. exigua. Meanwhile, the CXE would be a potential target for developing behavioral antagonists and pesticides against S. exigua" |
| Keywords: | "Animals Arthropod Antennae/enzymology Carboxylesterase/*genetics/metabolism In Situ Hybridization, Fluorescence Insect Proteins/*genetics/metabolism Spodoptera/*enzymology Spodoptera exigua antennae-enriched expression, general odorant-degrading enzyme ca;" |
| Notes: | "MedlineHe, Peng Zhang, Ya-Nan Yang, Ke Li, Zhao-Qun Dong, Shuang-Lin eng Research Support, Non-U.S. Gov't 2015/08/13 Pestic Biochem Physiol. 2015 Sep; 123:93-100. doi: 10.1016/j.pestbp.2015.03.009. Epub 2015 Mar 26" |
