| Title: | Membrane-displayed peptide ligand activates the pheromone response pathway in Saccharomyces cerevisiae |
| Author(s): | Hara K; Ono T; Kuroda K; Ueda M; |
| Address: | "Research Fellow of the Japan Society for the Promotion of Science, Sakyo-ku, Kyoto 606-8502, Japan" |
| ISSN/ISBN: | 1756-2651 (Electronic) 0021-924X (Linking) |
| Abstract: | "The budding yeast, Saccharomyces cerevisiae, is an attractive host for studying G protein-coupled receptors (GPCRs). We developed a system in which a peptide ligand specific for GPCR is displayed on yeast plasma membrane. The model system described here is based on yeast plasma membrane display of an analogue of alpha-factor, which is a peptide ligand for Ste2p, the GPCR that activates the yeast pheromone response pathway. alpha-Factor analogues, containing linkers of varying lengths and produced in yeast cells, became attached to the cell plasma membrane by linking to the glycosylphosphatidylinositol (GPI)-anchored plasma membrane protein Yps1p. We were able to demonstrate that an optimized alpha-factor analogue activated the pheromone response pathway in S. cerevisiae, as assessed by a fluorescent reporter assay. Furthermore, it was shown that linker length strongly influenced signalling pathway activation. To our knowledge, this is the first report documenting functional signalling by a plasma membrane-displayed ligand in S. cerevisiae" |
| Keywords: | "Cell Membrane/chemistry/*metabolism Ligands Peptides/chemistry/metabolism/*pharmacology Pheromones/*pharmacology Receptors, G-Protein-Coupled/antagonists & inhibitors/metabolism Saccharomyces cerevisiae/cytology/*drug effects/*metabolism Signal Transducti;" |
| Notes: | "MedlineHara, Keisuke Ono, Takuya Kuroda, Kouichi Ueda, Mitsuyoshi eng Research Support, Non-U.S. Gov't England 2012/03/13 J Biochem. 2012 May; 151(5):551-7. doi: 10.1093/jb/mvs027. Epub 2012 Mar 8" |
