| Title: | "Expression profile and functional characterization of odorant binding proteins in a forest pest, Dioryctria abietella (Lepidoptera: Pyralidae)" |
| Author(s): | Guo YR; Yin NN; Wu C; Yang ZX; Wang ZQ; Liu NY; |
| Address: | "Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China. Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China. Electronic address: naiyong.liu@swfu.edu.cn" |
| Journal Title: | Comp Biochem Physiol B Biochem Mol Biol |
| DOI: | 10.1016/j.cbpb.2023.110835 |
| ISSN/ISBN: | 1879-1107 (Electronic) 1096-4959 (Linking) |
| Abstract: | "In the forest ecosystem dominated by the Pinaceae plants, this boring pest Dioryctria abietella is subject to a variety of odorants derived from host and nonhost plants, in which olfactory-related proteins enriched in antennae are key behavioral modulators for the orientation of feeding and ovipositing hosts. Here, we addressed the odorant binding protein (OBP) gene family in D. abietella. Expression profiles revealed that the majority of OBPs were abundantly expressed in the antennae at a female-biased level. A male-antenna-biased DabiPBP1 was a strong candidate for detecting type I and type II pheromones of D. abitella female moths. Using a prokaryotic expression system combined with affinity chromatography, we harvested two antenna-dominant DabiOBPs. In the ligand-binding assays, the two DabiOBPs exhibited different odorant response spectra, as DabiOBP17 was tuned to most odorants with higher affinities compared to DabiOBP4. Of these, DabiOBP4 could strongly bind syringaldehyde and citral (dissociation constants (K(i)) < 14 muM). A floral volatile, benzyl benzoate (K(i) = 4.72 +/- 0.20 muM), was the best ligand for DabiOBP17. Remarkably, several green leaf volatiles were found to strongly interact with DabiOBP17 (K(i) < 8.5 muM), including Z3-hexenyl acetate, E2-hexenol, Z2-hexenal and E2-hexenal that may mediate a repellent response to D. abietella. Structural analyses of ligands revealed that the binding of the two DabiOBPs to odorants was associated with carbon-chain lengths and functional groups. Molecular simulations identified several key residues involved in the interactions of DabiOBPs and ligands, suggesting specific binding mechanisms. This study highlights olfactory roles of two antennal DabiOBPs in D. abietella, helping the identification of potentially behavioral compounds for the population control of this pest" |
| Keywords: | "Animals Odorants Ligands Ecosystem Hexobarbital/metabolism Insect Proteins/genetics/metabolism *Moths/genetics/metabolism *Receptors, Odorant/metabolism Forests Arthropod Antennae/metabolism Dioryctria abietella Ligand structure Ligand-binding assay Molec;" |
| Notes: | "MedlineGuo, Yu-Ruo Yin, Ning-Na Wu, Chun Yang, Zi-Xuan Wang, Zheng-Quan Liu, Nai-Yong eng England 2023/02/22 Comp Biochem Physiol B Biochem Mol Biol. 2023 Jun-Jul; 266:110835. doi: 10.1016/j.cbpb.2023.110835. Epub 2023 Feb 15" |
