| Title: | Cellular reactions to long-term volatile organic compound (VOC) exposures |
| Author(s): | Gostner JM; Zeisler J; Alam MT; Gruber P; Fuchs D; Becker K; Neubert K; Kleinhappl M; Martini S; Uberall F; |
| Address: | "Division of Medical Biochemistry, Biocenter, Medical University of Innsbruck, Austria. Bioenergy 2020+, Graz, Austria. Division of Biomedical Sciences, Warwick Medical School, University of Warwick, UK. Division of Biological Chemistry, Biocenter, Medical University of Innsbruck, Austria. ATLAS Biolabs GmbH, Berlin, Germany" |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Investigations of cellular processes initiated by volatile organic compounds (VOCs) are limited when modelling realistic long-term exposure scenarios at low concentrations. Exposure to indoor VOCs is associated with a range of adverse effects, but data on molecular changes at regulatory threshold limits are lacking. Activity analysis of VOC in vitro can be a valuable complement to inhalation toxicological evaluations. We developed an exposure platform that generates a stable VOC atmosphere and allows the exposure of cells for longer periods. Using formaldehyde as a model analyte, air-liquid interface cultured A549 lung epithelial cells were exposed to critical concentrations of 0.1 and 0.5 ppm for 3 days. Owing to the lack of known exposure biomarkers, we applied a genome-wide transcriptional analysis to investigate cellular responses at these sublethal concentrations. We demonstrate a minor overlap of differentially expressed transcripts for both treatment concentrations, which can be further analyzed for their use as exposure biomarkers. Moreover, distinct expression patterns emerge for 0.1 and 0.5 ppm formaldehyde exposure, which is reflected in significant enrichment of distinct biological processes. More specifically, metabolism of specific compound classes, lipid biosynthesis and lung-associated functions are affected by lower exposure levels and processes affecting proliferation and apoptosis dominate the higher exposure levels" |
| Keywords: | "A549 Cells/drug effects Air Pollutants/*analysis Air Pollution, Indoor Biomarkers/metabolism Cell Survival Environmental Exposure Formaldehyde/*adverse effects Gene Expression Profiling Gene Expression Regulation Humans Lipids/chemistry Lung/cytology/*dru;" |
| Notes: | "MedlineGostner, Johanna M Zeisler, Johannes Alam, Mohammad Tauqeer Gruber, Peter Fuchs, Dietmar Becker, Kathrin Neubert, Kerstin Kleinhappl, Markus Martini, Stefan Uberall, Florian eng T 703/FWF_/Austrian Science Fund FWF/Austria Research Support, Non-U.S. Gov't England 2016/12/03 Sci Rep. 2016 Dec 1; 6:37842. doi: 10.1038/srep37842" |
