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Chem Senses

Title:		Differential expression of SNMP-1 and SNMP-2 proteins in pheromone-sensitive hairs of moths
Author(s):		Forstner M; Gohl T; Gondesen I; Raming K; Breer H; Krieger J;
Address:		"Institute of Physiology (230), University of Hohenheim, Garbenstrasse 30, 70599 Stuttgart, Germany"
Journal Title:		Chem Senses
Year:		2008
Volume:		20080121
Issue:		3
Page Number:		291 - 299
DOI:		10.1093/chemse/bjm087
ISSN/ISBN:		0379-864X (Print) 0379-864X (Linking)
Abstract:		"In moths the detection of female-released sex pheromones involves hairlike structures on the male antenna. These long sensilla trichodea usually contain 2-3 chemosensory neurons accompanied by several supporting cells. Previous studies have shown that the pheromone-specific neurons are characterized by a 'sensory neuron membrane protein' (SNMP) which is homologous to the CD36 family and localized in the dendrite membrane. By employing the SNMP-2 sequence from Manduca sexta we have isolated cDNAs that encode SNMP-2 proteins from Heliothis virescens (HvirSNMP-2) and Antheraea polyphemus (ApolSNMP-2). To elucidate the topographic and cell type-specific expression of these SNMP subtypes, 2-color in situ hybridization experiments were performed with tissue sections through the male antennae. For H. virescens, a specific probe for the pheromone receptor HR13 was used to identify pheromone-responsive neurons. It was found that HvirSNMP-1 and HR13 were coexpressed in the same cells; in contrast, HvirSNMP-2 was not expressed in HR13 cells but rather in cells that surrounded the HR13 neurons, apparently the supporting cells. A corresponding expression pattern was also found for ApolSNMP-1 and ApolSNMP-2 on the antenna of male A. polyphemus. Our results indicate that SNMP-1s and SNMP-2s are differentially expressed in cells of pheromone-sensitive sensilla and suggest distinct functions for the 2 SNMP subtypes in the olfactory system"
Keywords:		"Animals *Gene Expression Profiling Gene Library In Situ Hybridization, Fluorescence Insect Proteins/*genetics Lepidoptera Male Manduca Membrane Proteins/*genetics Molecular Sequence Data Moths/*genetics Nerve Tissue Proteins/*genetics Neuroglia/*metabolis;"
Notes:		"MedlineForstner, Maike Gohl, Thomas Gondesen, Inga Raming, Klaus Breer, Heinz Krieger, Jurgen eng Research Support, Non-U.S. Gov't England 2008/01/23 Chem Senses. 2008 Mar; 33(3):291-9. doi: 10.1093/chemse/bjm087. Epub 2008 Jan 21"