« Previous AbstractDamaged-self recognition in common bean (Phaseolus vulgaris) shows taxonomic specificity and triggers signaling via reactive oxygen species (ROS)    Next AbstractDetermination of Volatile Metabolites in Vinegar by Stir Bar Sorptive Extraction-Gas Chromatography-Mass Spectrometry (SBSE-GC-MS) »

Brain Behav Immun

Title:		Extracellular self-DNA as a damage-associated molecular pattern (DAMP) that triggers self-specific immunity induction in plants
Author(s):		Duran-Flores D; Heil M;
Address:		"Departamento de Ingenieria Genetica, CINVESTAV-Irapuato, Irapuato, Guanajuato, Mexico. Departamento de Ingenieria Genetica, CINVESTAV-Irapuato, Irapuato, Guanajuato, Mexico. Electronic address: mheil@ira.cinvestav.mx"
Journal Title:		Brain Behav Immun
Year:		2018
Volume:		20171016
Issue:
Page Number:		78 - 88
DOI:		10.1016/j.bbi.2017.10.010
ISSN/ISBN:		1090-2139 (Electronic) 0889-1591 (Linking)
Abstract:		"Mammals sense self or non-self extracellular or extranuclear DNA fragments (hereinafter collectively termed eDNA) as indicators of injury or infection and respond with immunity. We hypothesised that eDNA acts as a damage-associated molecular pattern (DAMP) also in plants and that it contributes to self versus non-self discrimination. Treating plants and suspension-cultured cells of common bean (Phaseolus vulgaris) with fragmented self eDNA (obtained from other plants of the same species) induced early, immunity-related signalling responses such as H(2)O(2) generation and MAPK activation, decreased the infection by a bacterial pathogen (Pseudomonas syringae) and increased an indirect defence to herbivores (extrafloral nectar secretion). By contrast, non-self DNA (obtained from lima bean, Phaseolus lunatus, and Acacia farnesiana) had significantly lower or no detectable effects. Only fragments below a size of 700?ª+bp were active, and treating the eDNA preparation DNAse abolished its inducing effects, whereas treatment with RNAse or proteinase had no detectable effect. These findings indicate that DNA fragments, rather than small RNAs, single nucleotides or proteins, accounted for the observed effects. We suggest that eDNA functions a DAMP in plants and that plants discriminate self from non-self at a species-specific level. The immune systems of plants and mammals share multiple central elements, but further work will be required to understand the mechanisms and the selective benefits of an immunity response that is triggered by eDNA in a species-specific manner"
Keywords:		"Alarmins/*genetics/metabolism/physiology Cell-Free Nucleic Acids/genetics/immunology/*physiology DNA/immunology/metabolism Immunity, Innate/genetics MAP Kinase Signaling System/immunology Phaseolus/genetics/immunology Plants/genetics/*immunology Reactive;"
Notes:		"MedlineDuran-Flores, Dalia Heil, Martin eng Research Support, Non-U.S. Gov't Netherlands 2017/10/19 Brain Behav Immun. 2018 Aug; 72:78-88. doi: 10.1016/j.bbi.2017.10.010. Epub 2017 Oct 16"