« Previous AbstractDamage of brown planthopper (BPH) Nilaparvata lugens and rice leaf folder (LF) Cnaphalocrocis medinalis in parent plants lead to distinct resistance in ratoon rice    Next AbstractMeat quality and flavor compounds of soft-boiled chickens: effect of Chinese yellow-feathered chicken breed and slaughter age »

Chem Senses

Title:		Function and immunocytochemical localization of two novel odorant-binding proteins in olfactory sensilla of the scarab beetle Holotrichia oblita Faldermann (Coleoptera: Scarabaeidae)
Author(s):		Deng S; Yin J; Zhong T; Cao Y; Li K;
Address:		"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, West Yuanmingyuan Road, Beijing, China"
Journal Title:		Chem Senses
Year:		2012
Volume:		20110818
Issue:		2
Page Number:		141 - 150
DOI:		10.1093/chemse/bjr084
ISSN/ISBN:		1464-3553 (Electronic) 0379-864X (Linking)
Abstract:		"Odorant-binding proteins (OBPs) are found in both insects and vertebrates, and it is believed that they are involved in chemical communication. In this study, we identify and express 2 OBPs from the scarab beetle, Holotrichia oblita Faldermann (Coleoptera: Scarabaeidae). HoblOBP1 shows more similarities with other scarab beetle OBPs, whereas HoblOBP2 is more diverse. N-phenyl-1-naphthylamine (1-NPN) is used as a fluorescent probe in ligand-binding experiment, and results indicate that both HoblOBPs prefer plant volatiles to putative H. oblita sex pheromones. HoblOBP1 shows binding affinity to a wider range of test compounds, but HoblOBP2 displays more specific binding affinity. Cinnamaldehyde and 2,4-di-tert-butylphenol bind to HoblOBP1 can elicit strong electrophysiological responses of the antennae from female H. oblita adults, respectively. Methyl salicylate also shows good affinity to HoblOBP2 and it can elicit moderate electrophysiological responses. Although, beta-ionone is one of the ligands of the strongest binding, it elicits a weak electrophysiological response. In the immunocytochemical analysis, we observe that HoblOBP1 and HoblOBP2 are coexpressed in sensilla basiconica and placodea in both sexes"
Keywords:		1-Naphthylamine/analogs & derivatives/analysis Acrolein/analogs & derivatives/metabolism Amino Acid Sequence Animals Arthropod Antennae/*physiology Coleoptera Female Insect Proteins/genetics/*metabolism Male Molecular Sequence Data Norisoprenoids/metaboli;
Notes:		"MedlineDeng, Sisi Yin, Jiao Zhong, Tao Cao, Yazhong Li, Kebin eng Research Support, Non-U.S. Gov't England 2011/08/20 Chem Senses. 2012 Feb; 37(2):141-50. doi: 10.1093/chemse/bjr084. Epub 2011 Aug 18"