| Title: | "Bioproduction of (Z,E)-9,12-tetradecadienyl acetate (ZETA), the major pheromone component of Plodia, Ephestia, and Spodoptera species in yeast" |
| Author(s): | Ding BJ; Wang HL; Al-Saleh MA; Lofstedt C; Antony B; |
| Address: | "Department of Biology, Lund University, Lund, Sweden. Department of Plant Protection, King Saud University, Chair of Date Palm Research, Chemical Ecology and Functional Genomics Laboratory, College of Food and Agricultural Sciences, Riyadh, Saudi Arabia" |
| ISSN/ISBN: | 1526-4998 (Electronic) 1526-498X (Print) 1526-498X (Linking) |
| Abstract: | "BACKGROUND: (Z,E)-9,12-tetradecadienyl acetate (ZETA, Z9,E12-14:OAc) is a major sex pheromone component for many stored-product moth species. This pheromone is used worldwide for mating disruption, detection, monitoring, and mass trapping in raw and processed food storage facilities. In this study, we demonstrate the biological production of ZETA pheromone by engineered yeast Saccharomyces cerevisiae. RESULTS: We mined the pheromone gland transcriptome data of the almond moth, Ephestia (Cadra) cautella (Walker), to trace a novel E12 fatty acyl desaturase and expressed candidates heterologously in yeast and Sf9 systems. Furthermore, we demonstrated a tailor-made ZETA pheromone bioproduction in yeast through metabolic engineering using this E12 desaturase, in combination with three genes from various sources coding for a Z9 desaturase, a fatty acyl reductase, and an acetyltransferase, respectively. Electrophysiological assays (gas chromatography coupled to an electroantennographic detector) proved that the transgenic yeast-produced ZETA pheromone component elicits distinct antennal responses. CONCLUSION: The reconstructed biosynthetic pathway in yeast efficiently produces ZETA pheromone, leaves an undetectable level of biosynthetic intermediates, and paves the way for the economically competitive high-demand ZETA pheromone's bioproduction technology for high-value storage pest control" |
| Keywords: | "Animals *Moths/genetics Pheromones Saccharomyces cerevisiae/genetics *Sex Attractants Spodoptera (Z, E)-9, 12-tetradecadienyl acetate Saccharomyces cerevisiae bioproduction of pheromone mating disruption pest management pheromone;" |
| Notes: | "MedlineDing, Bao-Jian Wang, Hong-Lei Al-Saleh, Mohammed Ali Lofstedt, Christer Antony, Binu eng Deanship of Scientific Research, King Saud University/ King Abdul Aziz City for Science and Technology/ Swedish Research Council/ England 2021/11/14 Pest Manag Sci. 2022 Mar; 78(3):1048-1059. doi: 10.1002/ps.6716. Epub 2021 Dec 6" |
