| Title: | Identification of genes in the phenylalanine metabolic pathway by ectopic expression of a MYB transcription factor in tomato fruit |
| Author(s): | Dal Cin V; Tieman DM; Tohge T; McQuinn R; de Vos RC; Osorio S; Schmelz EA; Taylor MG; Smits-Kroon MT; Schuurink RC; Haring MA; Giovannoni J; Fernie AR; Klee HJ; |
| Address: | "University of Florida, Horticultural Sciences, Gainesville, Florida 32611-0690, USA" |
| ISSN/ISBN: | 1532-298X (Electronic) 1040-4651 (Print) 1040-4651 (Linking) |
| Abstract: | "Altering expression of transcription factors can be an effective means to coordinately modulate entire metabolic pathways in plants. It can also provide useful information concerning the identities of genes that constitute metabolic networks. Here, we used ectopic expression of a MYB transcription factor, Petunia hybrida ODORANT1, to alter Phe and phenylpropanoid metabolism in tomato (Solanum lycopersicum) fruits. Despite the importance of Phe and phenylpropanoids to plant and human health, the pathway for Phe synthesis has not been unambiguously determined. Microarray analysis of ripening fruits from transgenic and control plants permitted identification of a suite of coregulated genes involved in synthesis and further metabolism of Phe. The pattern of coregulated gene expression facilitated discovery of the tomato gene encoding prephenate aminotransferase, which converts prephenate to arogenate. The expression and biochemical data establish an arogenate pathway for Phe synthesis in tomato fruits. Metabolic profiling and (1)(3)C flux analysis of ripe fruits further revealed large increases in the levels of a specific subset of phenylpropanoid compounds. However, while increased levels of these human nutrition-related phenylpropanoids may be desirable, there were no increases in levels of Phe-derived flavor volatiles" |
| Keywords: | "Amino Acids, Dicarboxylic/chemistry/metabolism Cyclohexanecarboxylic Acids/chemistry/metabolism Cyclohexenes/chemistry/metabolism Fruit/chemistry/genetics/*metabolism *Gene Expression Regulation, Plant Humans Solanum lycopersicum/chemistry/*genetics/*meta;" |
| Notes: | "MedlineDal Cin, Valeriano Tieman, Denise M Tohge, Takayuki McQuinn, Ryan de Vos, Ric C H Osorio, Sonia Schmelz, Eric A Taylor, Mark G Smits-Kroon, Miriam T Schuurink, Robert C Haring, Michel A Giovannoni, James Fernie, Alisdair R Klee, Harry J eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2011/07/14 Plant Cell. 2011 Jul; 23(7):2738-53. doi: 10.1105/tpc.111.086975. Epub 2011 Jul 12" |
