| Title: | A phylogenomics approach to characterizing sensory neuron membrane proteins (SNMPs) in Lepidoptera |
| Author(s): | Zhang HJ; Xu W; Chen QM; Sun LN; Anderson A; Xia QY; Papanicolaou A; |
| Address: | "State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400715, China; CSIRO Food Futures Flagship, Canberra, ACT, 2601, Australia; CSIRO Ecosystem Sciences, Canberra, ACT, 2601, Australia. Agricultural Sciences, Murdoch University, Murdoch, WA, 6150, Australia. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400715, China. CSIRO Food Futures Flagship, Canberra, ACT, 2601, Australia. State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, 400715, China. Electronic address: xiaqy@swu.edu.cn. CSIRO Ecosystem Sciences, Canberra, ACT, 2601, Australia; Hawkesbury Institute for the Environment, Western Sydney University, Richmond, 2753, Australia. Electronic address: A.Papanicolaou@westernsydney.edu.au" |
| DOI: | 10.1016/j.ibmb.2020.103313 |
| ISSN/ISBN: | 1879-0240 (Electronic) 0965-1748 (Linking) |
| Abstract: | "Sensory neuron membrane proteins (SNMPs) play a critical role in the insect olfactory system but there is a deficit of functional studies beyond Drosophila. Here, we use a combination of available genome sequences, manual curation, genome and transcriptome data, phylogenetics, expression profiling and gene knockdown to investigate SNMP superfamily in various insect species with a focus on Lepidoptera. We curated 81 genes from 36 insect species and identified a novel lepidopteran SNMP gene family, SNMP3. Phylogenetic analysis shows that lepidopteran SNMP3, but not the previously annotated lepidopteran SNMP2, is the true homologue of the dipteran SNMP2. Digital expression, microarray and qPCR analyses show that the lepidopteran SNMP1 is specifically expressed in adult antennae. SNMP2 is widely expressed in multiple tissues while SNMP3 is specifically expressed in the larval midgut. Microarray analysis suggest SNMP3 may be involved in the silkworm immunity response to virus and bacterial infections. We functionally characterized SNMP1 in the silkworm using RNA interference (RNAi) and behavioral assays. Our results suggested that Bombyx mori SNMP1 is a functional orthologue of the Drosophila melanogaster SNMP1 and plays a critical role in pheromone detection. Split-ubiquitin yeast hybridization study shows that BmorSNMP1 has a protein-protein interaction with the pheromone receptor (BmorOR1), and the co-receptor (BmorOrco). Concluding, we propose a novel molecular model in which BmorOrco, BmorSNMP1 and BmorOR1 form a heteromer in the detection of the silkworm sex pheromone bombykol" |
| Keywords: | "Animals Butterflies/*genetics/metabolism Insect Proteins/*genetics/metabolism Membrane Proteins/*genetics/metabolism Moths/*genetics/metabolism Nerve Tissue Proteins/*genetics/metabolism Phylogeny Sensory Receptor Cells/metabolism Sequence Analysis, DNA S;" |
| Notes: | "MedlineZhang, Hui-Jie Xu, Wei Chen, Quan-Mei Sun, Le-Na Anderson, Alisha Xia, Qing-You Papanicolaou, Alexie eng Research Support, Non-U.S. Gov't England 2020/01/09 Insect Biochem Mol Biol. 2020 Mar; 118:103313. doi: 10.1016/j.ibmb.2020.103313. Epub 2020 Jan 3" |
