| Title: | Real-time measurement of quorum-sensing signal autoinducer 3OC6HSL by a FRET-based nanosensor |
| Address: | "Laboratory of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Meilong Road 130, Shanghai, 200237, China" |
| DOI: | 10.1007/s00449-013-1055-7 |
| ISSN/ISBN: | 1615-7605 (Electronic) 1615-7591 (Linking) |
| Abstract: | "Quorum sensing (QS) is involved in many important biological functions such as luminescence, antibiotic production, and biofilm formation. The autoinducer N-(3-oxo-hexanoyl)-L-homoserine lactone (3OC6HSL) plays a significant role in the QS system of the marine bacterium Vibrio fischeri. Tracing 3OC6HSL would be significant in studies related to QS signal transduction. Traditional detection of QS signaling molecules has relied on bacterial reporter strains and high-performance liquid chromatography, which are time consuming and have low sensitivity. Because 3OC6HSL binding to LuxR from V. fischeri causes a conformational change, we developed a genetically encoded biosensor based on Forster resonance energy transfer (FRET) by inserting LuxR between the FRET pair YFP/CFP. The detection limit of the sensor was 100 muM. We attained an optimized sensor with 70 % Deltaratio increase by screening different hydrophobic linkers, and demonstrated the feasibility of this sensor for visualizing 3OC6HSL both in vitro and in vivo" |
| Keywords: | 4-Butyrolactone/*analogs & derivatives/chemistry Aliivibrio fischeri/*growth & development Bacterial Proteins/*analysis Biosensing Techniques/*methods Fluorescence Resonance Energy Transfer Pheromones/chemistry *Quorum Sensing Repressor Proteins/*analysis; |
| Notes: | "MedlineZhang, Chang Ye, Bang-Ce eng Research Support, Non-U.S. Gov't Germany 2013/09/24 Bioprocess Biosyst Eng. 2014 May; 37(5):849-55. doi: 10.1007/s00449-013-1055-7. Epub 2013 Sep 22" |
