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Plant Signal Behav

Title:		Plasmid engineering of aphid alarm pheromone in tobacco seedlings affects the preference of aphids
Author(s):		Yu X; Jia D; Duan P;
Address:		"a School of Agricultural Engineering/Henan Collaborative Innovation Center of Water Security for Water Source Region of Mid-line of South-to-North Diversion Project , Nanyang Normal University , Nanyang , Henan , China"
Journal Title:		Plant Signal Behav
Year:		2019
Volume:		20190308
Issue:		5
Page Number:		e1588669 -
DOI:		10.1080/15592324.2019.1588669
ISSN/ISBN:		1559-2324 (Electronic) 1559-2316 (Print) 1559-2316 (Linking)
Abstract:		"Plants producing sufficient amount of aphid alarm pheromone by expressing (E)-beta-Farnesene (EbetaF) synthase gene may contribute to plant protection by reducing aphid populations. However, terpene biosynthesis varies among plant species and developmental stages. In the present study, volatile headspace analysis of tobacco seedlings with MabetaFS1 (an EbetaF synthase from the Asian peppermint Mentha asiatica) failed to generate EbetaF. We further targeted MabetaFS1 to the tobacco plastid, using a chloroplast targeting sequence, either with or without the AtFPS1 gene for the biosynthesis of the precursor farnesyl diphosphate. When both MabetaFS1 and AtFPS1 genes were targeted to the chloroplast, low levels of EbetaF were detected in stably transformed tobacco seedlings; resulting in specific repellence of the green peach aphid, Myzus persicae. These data indicate that redirecting the EbetaF biosynthetic pathway from its natural cytosolic location to the chloroplast is a valid strategy. This redirecting strategy may be very useful for other crop plants that do not naturally produce EbetaF or other repellent volatiles"
Keywords:		Animals Aphids/genetics/*metabolism Plasmids/genetics Seedlings/genetics/*metabolism Sesquiterpenes/metabolism Tobacco/genetics/*metabolism ()-beta-Farnesene synthase Tobacco seedling aphid repellence sesquiterpene;
Notes:		"MedlineYu, Xiudao Jia, Dianyong Duan, Pengfei eng Research Support, Non-U.S. Gov't 2019/03/09 Plant Signal Behav. 2019; 14(5):e1588669. doi: 10.1080/15592324.2019.1588669. Epub 2019 Mar 8"