| Title: | Post-Transcriptional Control of Mating-Type Gene Expression during Gametogenesis in Saccharomyces cerevisiae |
| Author(s): | Yeager R; Bushkin GG; Singer E; Fu R; Cooperman B; McMurray M; |
| Address: | "Department of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA. Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA. RNA Biosciences Initiative, School of Medicine, University of Colorado, Aurora, CO 80045, USA" |
| ISSN/ISBN: | 2218-273X (Electronic) 2218-273X (Linking) |
| Abstract: | "Gametogenesis in diploid cells of the budding yeast Saccharomyces cerevisiae produces four haploid meiotic products called spores. Spores are dormant until nutrients trigger germination, when they bud asexually or mate to return to the diploid state. Each sporulating diploid produces a mix of spores of two haploid mating types, a and alpha. In asexually dividing haploids, the mating types result from distinct, mutually exclusive gene expression programs responsible for production of mating pheromones and the receptors to sense them, all of which are silent in diploids. It was assumed that spores only transcribe haploid- and mating-type-specific genes upon germination. We find that dormant spores of each mating type harbor transcripts representing all these genes, with the exception of Mata1, which we found to be enriched in a spores. Mata1 transcripts, from a rare yeast gene with two introns, were mostly unspliced. If the retained introns reflect tethering to the MATa locus, this could provide a mechanism for biased inheritance. Translation of pheromones and receptors were repressed at least until germination. We find antisense transcripts to many mating genes that may be responsible. These findings add to the growing number of examples of post-transcriptional regulation of gene expression during gametogenesis" |
| Keywords: | "*Gametogenesis *Gene Expression Regulation Homeodomain Proteins/*metabolism Ploidies Repressor Proteins/*metabolism Saccharomyces cerevisiae/*metabolism Saccharomyces cerevisiae Proteins/*metabolism Spores, Fungal/*metabolism antisense budding yeast gamet;" |
| Notes: | "MedlineYeager, Randi Bushkin, G Guy Singer, Emily Fu, Rui Cooperman, Benjamin McMurray, Michael eng T32GM008730/NH/NIH HHS/ F32GM108201/NH/NIH HHS/ T32 GM136444/GM/NIGMS NIH HHS/ R01GM035010/NH/NIH HHS/ P30CA046934/NH/NIH HHS/ Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. Switzerland 2021/08/28 Biomolecules. 2021 Aug 17; 11(8):1223. doi: 10.3390/biom11081223" |
