| Title: | Transcriptomic profiling of the flower scent biosynthesis pathway of Cymbidium faberi Rolfe and functional characterization of its jasmonic acid carboxyl methyltransferase gene |
| Author(s): | Xu Q; Wang S; Hong H; Zhou Y; |
| Address: | "Center of Applied Biotechnology, Wuhan University of Bioengineering, Wuhan, 430415, People's Republic of China. College of Bioscience and Biotechnology, Wuhan University of Bioengineering, Wuhan, 430415, People's Republic of China. Present Address: Hainan Key Laboratory for the Sustainable Utilization of Tropical Bioresources, College of Agriculture, Hainan University, Haikou, 570228, People's Republic of China. Center of Applied Biotechnology, Wuhan University of Bioengineering, Wuhan, 430415, People's Republic of China. ripplet0931@hotmail.com. College of Bioscience and Biotechnology, Wuhan University of Bioengineering, Wuhan, 430415, People's Republic of China. ripplet0931@hotmail.com" |
| DOI: | 10.1186/s12864-019-5501-z |
| ISSN/ISBN: | 1471-2164 (Electronic) 1471-2164 (Linking) |
| Abstract: | "BACKGROUND: Cymbidium faberi, one of the most famous oriental orchids, has a distinct flower scent, which increases its economic value. However, the molecular mechanism of the flower scent biosynthesis was unclear prior to this study. Methyl jasmonate (MeJA) is one of the main volatile organic compounds (VOC) produced by the flowers of C. faberi. In this study, unigene 79,363 from comparative transcriptome analysis was selected for further investigation. RESULTS: A transcriptome comparison between blooming and withered flowers of C. faberi yielded a total of 9409 differentially expressed genes (DEGs), 558 of which were assigned to 258 pathways. The top ten pathways included alpha-linolenic acid metabolism, pyruvate metabolism and fatty acid degradation, which contributed to the conversion of alpha-linolenic acid to MeJA. One of the DEGs, jasmonic acid carboxyl methyltransferase (CfJMT, Unigene 79,363) was highly expressed in the blooming flower of C. faberi, but was barely detected in leaves and roots. Although the ectopic expression of CfJMT in tomato could not increase the MeJA content, the expression levels of endogenous MeJA biosynthesis genes were influenced, especially in the wound treatment, indicating that CfJMT may participate in the response to abiotic stresses. CONCLUSION: This study provides a basis for elucidating the molecular mechanism of flower scent biosynthesis in C. faberi, which is beneficial for the genetically informed breeding of new cultivars of the economically valuable oriental orchids" |
| Keywords: | "Flowers/*metabolism *Gene Expression Profiling Methyltransferases/*genetics *Odorants Orchidaceae/enzymology/*genetics/*metabolism/physiology Promoter Regions, Genetic/genetics Stress, Physiological Up-Regulation Flower fragrance Jasmonic acid carboxyl me;" |
| Notes: | "MedlineXu, Qi Wang, Songtai Hong, Huazhu Zhou, Yin eng 31700626/National Natural Science Foundation of China/ B2016304/Science and Technology Research Project of the Education Department of Hubei province/ England 2019/02/13 BMC Genomics. 2019 Feb 11; 20(1):125. doi: 10.1186/s12864-019-5501-z" |
