| Title: | Urinary Volatile Terpenes Analyzed by Gas Chromatography-Mass Spectrometry to Monitor Breast Cancer Treatment Efficacy in Mice |
| Author(s): | Woollam M; Teli M; Liu S; Daneshkhah A; Siegel AP; Yokota H; Agarwal M; |
| Address: | "Integrated Nanosystems Development Institute, Indiana University-Purdue University Indianapolis, Indianapolis 46202, Indiana, United States. Department of Chemistry and Chemical Biology, Indiana University-Purdue University Indianapolis, Indianapolis 46202, Indiana, United States. Department of Biomedical Engineering, Indiana University-Purdue University Indianapolis Indianapolis 46202, Indiana, United States. Biomechanics and Biomaterials Research Center, Indianapolis 46202, Indiana, United States. Department of Mechanical Engineering and Energy, Indiana University-Purdue University Indianapolis, Indianapolis 46202, Indiana, United States" |
| DOI: | 10.1021/acs.jproteome.9b00722 |
| ISSN/ISBN: | 1535-3907 (Electronic) 1535-3893 (Linking) |
| Abstract: | "Urinary volatile terpene (VT) levels are significantly altered with induced models of breast cancer in mice. The question arises whether VTs can detect the efficacy of antitumor treatments. BALB/c mice were injected with 4T1.2 murine tumor cells in the mammary pad or iliac artery to model localized breast cancer and induced bone metastasis. The effect of two dopaminergic antitumor agents was tested by conventional histology and altered VT levels. The headspace of urine specimens was analyzed by gas chromatography-mass spectrometry. In the localized model, the statistical significance (p < 0.05) was identified for 26% of VTs, and in the metastasis model, 19% of VTs. The authors discovered separate VT panels classifying localized/control [area under the curve (AUC) = 1.0] and metastasis/control (AUC = 0.98). Treatment samples were tested using these panels, which showed that mice treated with either agent were statistically significantly different from cancer samples, which is consistent with conventional analysis" |
| Keywords: | "Animals Gas Chromatography-Mass Spectrometry Mice Mice, Inbred BALB C *Neoplasms Solid Phase Microextraction Terpenes *Volatile Organic Compounds/analysis area under the receiver operator characteristic (AUROC) gas chromatography (GC) leave one out cross-;" |
| Notes: | "MedlineWoollam, Mark Teli, Meghana Liu, Shengzhi Daneshkhah, Ali Siegel, Amanda P Yokota, Hiroki Agarwal, Mangilal eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2020/04/02 J Proteome Res. 2020 May 1; 19(5):1913-1922. doi: 10.1021/acs.jproteome.9b00722. Epub 2020 Apr 13" |
