| Title: | Unveiling sugarcane defense response to Mythimna separata herbivory by a combination of transcriptome and metabolic analyses |
| Author(s): | Wang YR; Zhang JS; Wang R; Hou YM; Fu HA; Xie Y; Gao SJ; Wang JD; |
| Address: | "National Engineering Research Center of Sugarcane, Fujian Agricultural and Forestry University, Fuzhou, P. R. China. State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Key Lab of Biopesticide and Chemical Biology, Ministry of Education & Fujian Province Key Laboratory of Insect Ecology, College of Plant Protection, Fujian Agricultural and Forestry University, Fuzhou, P. R. China. Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, P. R. China" |
| ISSN/ISBN: | 1526-4998 (Electronic) 1526-498X (Linking) |
| Abstract: | "BACKGROUND: Sugarcane is the most important sugar crop in the world. Like other crops, sugarcane suffers from herbivorous insect attack. The oriental armyworm Mythimna separata is a devastating pest of various crops in northeast Asia and an outbreak of this pest can result in substantial yield loss for sugarcane. However, the plant defense response situation is widely acquisition in model crops, but there is little information about how sugarcane plants defend themselves against this herbivore at the molecular and biochemical levels. RESULTS: We combined transcriptome and metabolomic analysis to investigate the changes in gene expression and metabolic processes that occurred in sugarcane plants after continuous feeding by M. separata larvae for 12 and 24 h. We identified 13 662 genes and 55 metabolites that were differentially regulated in sugarcane plants fed on by M. separata. The genes involved in phytohormones, transcription factors, and kinase-related were activated and metabolism compounds such as carbohydrate, amino acid, ferulic substances and glutathione were detected regulated in sugarcane defense response. Comparable analyses showed a close correspondence relationship among pathways of phenylalanine metabolism, phenylpropanoid biosynthesis, and flavonoid biosynthesis in transcript and metabolite profiles. Furthermore, a bioassay experiment was conducted to test the influence of up-regulated metabolites on M. separata growth and found chlorogenic acid had a lethal effect. CONCLUSION: The results of our study greatly enhanced the understanding of the sugarcane-induced defense response mechanism against herbivore infestation at the transcriptional and metabolic levels. Also make contributions to provide clues for development of green pest control method. (c) 2021 Society of Chemical Industry" |
| Keywords: | Animals Herbivory Larva/genetics *Moths/genetics *Saccharum/genetics Transcriptome Mythimna separata RNA sequencing metabolism phenylpropanoids sugarcane; |
| Notes: | "MedlineWang, Ya-Ru Zhang, Jia-Song Wang, Ran Hou, You-Ming Fu, Hu-Aying Xie, Yuan Gao, San-Ji Wang, Jin-da eng 2018YFD201100/National Key R&D Program of China/ 31601363/NSFC project/ KFA17523A/Special Technology Innovation Funding of Fujian Agriculture and Forestry University/ CARS-17/the Sugar Crop Research System, CARS/ England 2021/06/24 Pest Manag Sci. 2021 Oct; 77(10):4799-4809. doi: 10.1002/ps.6526. Epub 2021 Jul 10" |
