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BMC Chem


Title:Optimization of SPME-GC-MS and characterization of floral scents from Aquilegia japonica and A. amurensis flowers
Author(s):Wang HY; Zhang W; Dong JH; Wu H; Wang YH; Xiao HX;
Address:"Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, Changchun, 130024, China. Faculty of Chemistry, Northeast Normal University, Changchun, 130024, China. Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, Changchun, 130024, China. xiaohx771@nenu.edu.cn"
Journal Title:BMC Chem
Year:2021
Volume:20210422
Issue:1
Page Number:26 -
DOI: 10.1186/s13065-021-00754-1
ISSN/ISBN:2661-801X (Electronic) 2661-801X (Linking)
Abstract:"BACKGROUND: The floral scents of plants play a key role in plant reproduction through the communication between plants and pollinators. Aquilegia as a model species for studying evolution, however, there have been few studies on the floral scents and relationships between floral scents and pollination for Aquilegia taxa. METHODS: In this study, three types of solid-phase micro-extraction (SPME) fiber coatings (DVB/PDMS, CAR/PDMS, DVB/CAR/PDMS) were evaluated for their performance in extracting volatile organic compounds (VOCs) from flowers of Aquilegia amurensis, which can contribute to the future studies of elucidating the role of floral scents in the pollination process. RESULTS: In total, 55 VOCs were identified, and among them, 50, 47 and 45 VOCs were extracted by the DVB/CAR/PDMS fiber, CAR/PDMS fiber and DVB/PDMS fibers, respectively. Only 30 VOCs were detected in A. japonica taxa. Furthermore, the relative contents of 8 VOCs were significant different (VIP > 1 and p < 0.05) between the A. amurensis and A. japonica. CONCLUSIONS: The results can be applied in new studies of the relationships between the chemical composition of floral scents and the processes of attraction of pollinator. It may provide new ideas for rapid evolution and frequent interspecific hybridization of Aquilegia"
Keywords:Columbines Gc-ms Northeast China Spme VOCs;
Notes:"PubMed-not-MEDLINEWang, Hua-Ying Zhang, Wei Dong, Jian-Hua Wu, Hao Wang, Yuan-Hong Xiao, Hong-Xing eng Switzerland 2021/04/24 BMC Chem. 2021 Apr 22; 15(1):26. doi: 10.1186/s13065-021-00754-1"

 
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