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Brain Struct Funct

Title:		"Structural, morphometric and immunohistochemical study of the rabbit accessory olfactory bulb"
Author(s):		Villamayor PR; Cifuentes JM; Quintela L; Barcia R; Sanchez-Quinteiro P;
Address:		"Department of Anatomy, Animal Production and Clinical Veterinary Sciences, Faculty of Veterinary, University of Santiago de Compostela, Av Carballo Calero s/n, 27002, Lugo, Spain. Department of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela, Lugo, Spain. Department of Biochemistry and Molecular Biology, Faculty of Veterinary, University of Santiago de Compostela, Lugo, Spain. Department of Anatomy, Animal Production and Clinical Veterinary Sciences, Faculty of Veterinary, University of Santiago de Compostela, Av Carballo Calero s/n, 27002, Lugo, Spain. pablo.sanchez@usc.es"
Journal Title:		Brain Struct Funct
Year:		2020
Volume:		20191204
Issue:		1
Page Number:		203 - 226
DOI:		10.1007/s00429-019-01997-4
ISSN/ISBN:		1863-2661 (Electronic) 1863-2653 (Linking)
Abstract:		"The accessory olfactory bulb (AOB) is the first neural integrative centre of the vomeronasal system (VNS), which is associated primarily with the detection of semiochemicals. Although the rabbit is used as a model for the study of chemocommunication, these studies are hampered by the lack of knowledge regarding the topography, lamination, and neurochemical properties of the rabbit AOB. To fill this gap, we have employed histological stainings: lectin labelling with Ulex europaeus (UEA-I), Bandeiraea simplicifolia (BSI-B4), and Lycopersicon esculentum (LEA) agglutinins, and a range of immunohistochemical markers. Anti-G proteins Galphai2/Galphao, not previously studied in the rabbit AOB, are expressed following an antero-posterior zonal pattern. This places Lagomorpha among the small groups of mammals that conserve a double-path vomeronasal reception. Antibodies against olfactory marker protein (OMP), growth-associated protein-43 (GAP-43), glutaminase (GLS), microtubule-associated protein-2 (MAP-2), glial fibrillary-acidic protein (GFAP), calbindin (CB), and calretinin (CR) characterise the strata and the principal components of the BOA, demonstrating several singular features of the rabbit AOB. This diversity is accentuated by the presence of a unique organisation: four neuronal clusters in the accessory bulbar white matter, two of them not previously characterised in any species (the gamma and delta groups). Our morphometric study of the AOB has found significant differences between sexes in the numerical density of principal cells, with larger values in females, a pattern completely opposite to that found in rats. In summary, the rabbit possesses a highly developed AOB, with many specific features that highlight the significant role played by chemocommunication among this species"
Keywords:		"Animals Cell Count Female Immunohistochemistry Male Mice, Inbred BALB C Neurons/*cytology Olfactory Bulb/*cytology/metabolism Rabbits Sex Characteristics Vomeronasal Organ/*cytology Accessory olfactory bulb Morphometry Sexual dimorphism Vomeronasal system;"
Notes:		"MedlineVillamayor, Paula R Cifuentes, Jose Manuel Quintela, Luis Barcia, Ramiro Sanchez-Quinteiro, Pablo eng 1551-8179/University of Santiago de Compostela/ Germany 2019/12/06 Brain Struct Funct. 2020 Jan; 225(1):203-226. doi: 10.1007/s00429-019-01997-4. Epub 2019 Dec 4"