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Probiotics Antimicrob Proteins

Title:		Safety of Lactobacillus plantarum ST8Sh and Its Bacteriocin
Author(s):		Todorov SD; Perin LM; Carneiro BM; Rahal P; Holzapfel W; Nero LA;
Address:		"Veterinary Department, Universidade Federal de Vicosa, Campus UFV, Vicosa, Minas Gerais, 36570-900, Brazil. slavi310570@abv.bg. Veterinary Department, Universidade Federal de Vicosa, Campus UFV, Vicosa, Minas Gerais, 36570-900, Brazil. Departamento de Engenharia e Tecnologia de Alimentos, UNESP - Universidade Estadual Paulista, Instituto de Biociencias, Letras e Ciencias Exatas, Sao Jose do Rio Preto, SP, Brazil. Instituto de Ciencias Exatas e Naturais, Universidade Federal de Mato Grosso, Rondonopolis, MT, Brazil. GEE, Handong Global University, Pohang, South Korea"
Journal Title:		Probiotics Antimicrob Proteins
Year:		2017
Volume:		9
Issue:		3
Page Number:		334 - 344
DOI:		10.1007/s12602-017-9260-3
ISSN/ISBN:		1867-1314 (Electronic) 1867-1306 (Linking)
Abstract:		"Total DNA extracted from Lb. plantarum ST8Sh was screened for the presence of more than 50 genes related to production of biogenic amines (histidine decarboxylase, tyrosine decarboxylase, and ornithine decarboxylase), virulence factors (sex pheromones, gelatinase, cytolysin, hyaluronidase, aggregation substance, enterococcal surface protein, endocarditis antigen, adhesion of collagen, integration factors), and antibiotic resistance (vancomycin, tetracycline, erythromycin, gentamicin, chloramphenicol, bacitracin). Lb. plantarum ST8Sh showed a low presence of virulence genes. Only 13 genes were detected (related to sex pheromones, aggregation substance, adhesion of collagen, tetracycline, gentamicin, chloramphenicol, erythromycin, but not to vancomycin, and bacitracin) and may be considered as indication of safety for application in fermented food products. In addition, interaction between Lb. plantarum ST8Sh and drugs from different groups were determined in order to establish possible application of the strain in combination with commercial drugs. Cytotoxicity of the semi-purified bacteriocins produced by Lb. plantarum ST8Sh was depended on applied concentration-highly cytotoxic when applied at 25 mug/mL and no cytotoxicity at 5 mug/mL"
Keywords:		"Bacteriocins/*biosynthesis Biogenic Amines/biosynthesis Cell Line, Tumor DNA, Bacterial/isolation & purification Drug Resistance, Multiple, Bacterial/*genetics Enterococcus faecalis *Genes, Bacterial Humans Lactobacillus plantarum/genetics/*metabolism Lis;"
Notes:		"MedlineTodorov, Svetoslav Dimitrov Perin, Luana M Carneiro, Bruno M Rahal, Paula Holzapfel, Wilhelm Nero, Luis Augusto eng Research Support, Non-U.S. Gov't 2017/02/25 Probiotics Antimicrob Proteins. 2017 Sep; 9(3):334-344. doi: 10.1007/s12602-017-9260-3"