| Title: | Phytoplasma SAP11 alters 3-isobutyl-2-methoxypyrazine biosynthesis in Nicotiana benthamiana by suppressing NbOMT1 |
| Author(s): | Tan CM; Li CH; Tsao NW; Su LW; Lu YT; Chang SH; Lin YY; Liou JC; Hsieh LC; Yu JZ; Sheue CR; Wang SY; Lee CF; Yang JY; |
| Address: | "Institute of Biochemistry, National Chung Hsing University, Taichung 402, Taiwan Ph.D. Program in Microbial Genomics, National Chung Hsing University and Academia Sinica, Taiwan. Institute of Biochemistry, National Chung Hsing University, Taichung 402, Taiwan. Department of Forestry, National Chung Hsing University, Taichung 402, Taiwan. Department of Chemistry, National Chung Hsing University, Taichung 402, Taiwan. Institute of Genomics and Bioinformatics, National Chung Hsing University, Taichung 402, Taiwan. Department of Applied Zoology, Agricultural Research Institute, Taichung 413, Taiwan. Department of Life Sciences, National Chung Hsing University, Taichung 402, Taiwan. Institute of Biochemistry, National Chung Hsing University, Taichung 402, Taiwan Ph.D. Program in Microbial Genomics, National Chung Hsing University and Academia Sinica, Taiwan Institute of Biotechnology, National Chung Hsing University, Taichung 402, Taiwan NCHU-UCD Plant and Food Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan jyang@nchu.edu.tw" |
| ISSN/ISBN: | 1460-2431 (Electronic) 0022-0957 (Print) 0022-0957 (Linking) |
| Abstract: | "Phytoplasmas are bacterial phytopathogens that release virulence effectors into sieve cells and act systemically to affect the physiological and morphological state of host plants to promote successful pathogenesis. We show here that transgenic Nicotiana benthamiana lines expressing the secreted effector SAP11 from Candidatus Phytoplasma mali exhibit an altered aroma phenotype. This phenomenon is correlated with defects in the development of glandular trichomes and the biosynthesis of 3-isobutyl-2-methoxypyrazine (IBMP). IBMP is a volatile organic compound (VOC) synthesized by an O-methyltransferase, via a methylation step, from a non-volatile precursor, 3-isobutyl-2-hydroxypyrazine (IBHP). Based on comparative and functional genomics analyses, NbOMT1, which encodes an O-methyltransferase, was found to be highly suppressed in SAP11-transgenic plants. We further silenced NbOMT1 through virus-induced gene silencing and demonstrated that this enzyme influenced the accumulation of IBMP in N. benthamiana In vitro biochemical analyses also showed that NbOMT1 can catalyse IBHP O-methylation in the presence of S-adenosyl-L-methionine. Our study suggests that the phytoplasma effector SAP11 has the ability to modulate host VOC emissions. In addition, we also demonstrated that SAP11 destabilized TCP transcription factors and suppressed jasmonic acid responses in N. benthamiana These findings provide valuable insights into understanding how phytoplasma effectors influence plant volatiles" |
| Keywords: | "Blotting, Western Methyltransferases/genetics/*metabolism Phylogeny Phytoplasma/*metabolism Plant Proteins/*metabolism Plants, Genetically Modified Pyrazines/*metabolism Reverse Transcriptase Polymerase Chain Reaction Tobacco/metabolism/*microbiology Tric;" |
| Notes: | "MedlineTan, Choon Meng Li, Chia-Hua Tsao, Nai-Wen Su, Li-Wen Lu, Yen-Ting Chang, Shu Heng Lin, Yi Yu Liou, Jyun-Cyuan Hsieh, Li-Ching Yu, Jih-Zu Sheue, Chiou-Rong Wang, Sheng-Yang Lee, Chin-Fa Yang, Jun-Yi eng Research Support, Non-U.S. Gov't England 2016/06/10 J Exp Bot. 2016 Jul; 67(14):4415-25. doi: 10.1093/jxb/erw225. Epub 2016 Jun 8" |
