| Title: | Proteome mapping of the Drosophila melanogaster male reproductive system |
| Address: | "Drosophila Genetic Resource Center, Kyoto Institute of Technology, Saga Ippongi-cho, Ukyo-ku, Kyoto, Japan" |
| ISSN/ISBN: | 1615-9861 (Electronic) 1615-9853 (Linking) |
| Abstract: | "The fruit fly Drosophila melanogaster is an excellent model organism for studying insect reproductive biology. Although the gene expression profiles of both male and female reproductive organs have been studied in detail, their proteomic profiles and functional characteristics largely remained to be clarified. In this study, we conducted proteome mapping of the male internal reproductive organs using 2-DE. We identified a total of 440 protein components from gels of the male reproductive organs (testis, seminal vesicle, accessory gland, ejaculatory duct, and ejaculatory bulb). A number of proteins associated with odorant/pheromone-binding, lipid metabolism, proteolysis, and antioxidation were expressed tissue specifically in the male reproductive system. Based on our proteomic data set, we constructed reference proteome maps of the reproductive organs, which will provide valuable information toward a comprehensive understanding of Drosophila reproduction" |
| Keywords: | "Animals Drosophila melanogaster/*physiology Electrophoresis, Gel, Two-Dimensional Genitalia, Male/anatomy & histology/chemistry/metabolism Insect Proteins/*analysis/metabolism Male Models, Biological *Peptide Mapping Proteomics Seminal Plasma Proteins/*an;" |
| Notes: | "MedlineTakemori, Nobuaki Yamamoto, Masa-Toshi eng Research Support, Non-U.S. Gov't Germany 2009/04/04 Proteomics. 2009 May; 9(9):2484-93. doi: 10.1002/pmic.200800795" |
