| Title: | Studies on reproductive strategies of Vitex negundo L. var. heterophylla (Franch.) Rehder (Lamiaceae) based on morphological characteristics and SSR markers |
| Author(s): | Sun X; Wang F; Cui R; Liu X; Li X; Dong J; Sun L; Qin S; Wang R; Zheng P; Wang H; |
| Address: | Institute of Ecology and Biodiversity School of Life Sciences Shandong University Qingdao China. Shandong Provincial Engineering and Technology Research Center for Vegetation Ecology Shandong University Qingdao China. Qingdao Forest Ecology Research Station of National Forestry and Grassland Administration Shandong University Qingdao China |
| ISSN/ISBN: | 2045-7758 (Print) 2045-7758 (Electronic) 2045-7758 (Linking) |
| Abstract: | "Vitex negundo L. var. heterophylla (Franch.) Rehder (Lamiaceae) is an important tree species for soil and water conservation, yet the reproductive ecology of this species remains to be elucidated. To investigate the reproductive traits of V. negundo var. heterophylla, the phenology, morphological characteristics (a suite of characters was assessed: floral morphology, nectar production, pollen viability, and stigma receptivity) and mating system of this species were systematically revealed for the first time in this study. Phenological observations, morphological measurements, and nectar production analysis were conducted during anthesis. Pollen viability and stigma receptivity at different flowering stages were measured by biochemical methods. Finally, genetic analysis based on SSR markers was used to reveal the mating system; outcrossing index and pollen-ovule ratio were also calculated to help analysis. V. negundo var. heterophylla showed several obvious characteristics of outcrossing, such as abundant and attractive flowers, secreting nectar, and emitting scent. In addition, mechanisms such as homogamy and a short anther-stigma distance that can promote self-fertilization were also identified in this species. The coexistence of selfing and outcrossing characteristics demonstrates a predominantly outcrossed mixed mating system (outcrossing rate, t = 95%). The scientific information provided by this study may contribute to conservation of V. negundo var. heterophylla from a reproductive perspective" |
| Keywords: | SSR markers Vitex negundo var.heterophylla mating system morphological characteristics reproductive strategies; |
| Notes: | "PubMed-not-MEDLINESun, Xiaohan Wang, Feng Cui, Rong Liu, Xiao Li, Xiangxiang Dong, Jibin Sun, Lu Qin, Siqi Wang, Renqing Zheng, Peiming Wang, Hui eng England 2020/07/02 Ecol Evol. 2020 Apr 22; 10(12):5270-5280. doi: 10.1002/ece3.6271. eCollection 2020 Jun" |
