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Neuroscience

Title:		Signal transduction and gene expression in cultured accessory olfactory bulb neurons
Author(s):		Skinner CB; Upadhya SC; Smith TK; Turner CP; Hegde AN;
Address:		"Department of Neurobiology and Anatomy, Wake Forest University Health Sciences, Medical Center Boulevard, Winston-Salem, NC 27157, USA"
Journal Title:		Neuroscience
Year:		2008
Volume:		20080916
Issue:		2
Page Number:		340 - 348
DOI:		10.1016/j.neuroscience.2008.09.016
ISSN/ISBN:		0306-4522 (Print) 0306-4522 (Linking)
Abstract:		"Glutamate and norepinephrine (NE) are believed to mediate the long-lasting synaptic plasticity in the accessory olfactory bulb (AOB) that underlies pheromone recognition memory. The mechanisms by which these neurotransmitters bring about the synaptic changes are not clearly understood. In order to study signals that mediate synaptic plasticity in the AOB, we used AOB neurons in primary culture as a model system. Because induction of pheromone memory requires coincident glutamatergic and noradrenergic input to the AOB, and requires new protein synthesis, we reasoned that glutamate and NE must induce gene expression in the AOB. We used a combination of agonists that stimulate alpha1 and alpha2 adrenergic receptors in combination with N-methyl-d-aspartic acid and tested expression of the immediate-early gene (IEG) c-Fos. We found that the glutamatergic and noradrenergic stimulation caused significant induction of c-Fos mRNA and protein. Induction of c-Fos was significantly reduced in the presence of inhibitors of protein kinase C, mitogen-activated protein kinase (MAPK) and phospholipase C. These results suggest that glutamate and NE induce gene expression in the AOB through a signaling pathway mediated by protein kinase C and MAPK"
Keywords:		"Adrenergic alpha-Agonists/pharmacology Age Factors Animals Animals, Newborn Cell Survival Cells, Cultured Enzyme Inhibitors/pharmacology Female Gene Expression/drug effects/*physiology Gene Expression Regulation, Developmental/drug effects/physiology Imid;Neuroscience;"
Notes:		"MedlineSkinner, C B Upadhya, S C Smith, T K Turner, C P Hegde, A N eng T32 DC000057/DC/NIDCD NIH HHS/ T32 DC000057-09/DC/NIDCD NIH HHS/ T32 DC000057-10/DC/NIDCD NIH HHS/ Z01 DC000057/Intramural NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2008/10/14 Neuroscience. 2008 Nov 19; 157(2):340-8. doi: 10.1016/j.neuroscience.2008.09.016. Epub 2008 Sep 16"