| Title: | Tolerance of a sulfidogenic sludge to trichloroethylene at microcosms level as a basis for a long-term operation of reactors designed for its biodegradation |
| Author(s): | Santana-Santos MA; Ordaz A; Jan-Roblero J; Bastida Gonzalez F; Zarate Segura PB; Guerrero-Barajas C; |
| Address: | "a Laboratorio de Biotecnologia Ambiental, Departamento de Bioprocesos , Unidad Profesional Interdisciplinaria de Biotecnologia, Instituto Politecnico Nacional , Mexico City 07340 , Mexico. b Escuela de Ingenieria y Ciencias , Instituto Tecnologico y de Estudios Superiores de Monterrey, Campus Estado de Mexico, Carretera Lago de Guadalupe Km 3.5, Margarita Maza de Juarez, Atizapan de Zaragoza , Estado de Mexico , Mexico. c Departamento de Microbiologia, Escuela Nacional de Ciencias Biologicas , Instituto Politecnico Nacional, Prol. de Carpio y Plan de Ayala s/n, Col. Santo Tomas , Mexico City , Mexico. d Laboratorio de Biologia Molecular, Laboratorio Estatal de Salud Publica del Estado de Mexico. Paseo Tollocan s/n, Col. La Moderna de la Cruz , Estado de Mexico , Toluca , Mexico. e Laboratorio de Medicina Traslacional , Escuela Superior de Medicina. Instituto Politecnico Nacional. Plan de San Luis y Diaz Miron s/n, Col. Santo Tomas , Mexico City , Mexico" |
| Journal Title: | J Environ Sci Health A Tox Hazard Subst Environ Eng |
| DOI: | 10.1080/10934529.2019.1567157 |
| ISSN/ISBN: | 1532-4117 (Electronic) 1093-4529 (Linking) |
| Abstract: | "Trichloroethylene (TCE) is known as a toxic organic compound found as a pollutant in water streams around the world. The ultimate goal of the present work was to determine the TCE concentration that would be feasible to biodegrade on a long-term basis by a sulfidogenic sludge while maintaining sulfate reducing activity (SRA). Microcosms were prepared with sulfidogenic sludge obtained from a stabilized sulfidogenic UASB and amended with different TCE concentrations (100-300 microM) and two different proportions of volatile fatty acids (VFA) acetate, propionate and butyrate at COD of 2.5:1:1 and 1:1:1, respectively to evaluate the tolerance of the sludge. The overall results suggested that the continuous exposure of the microorganisms to TCE leads to inhibition of SRA; nonetheless, the SRA can be recovered after adequate supplementation of carbon sources and sulfate. The most suitable TCE concentration to operate on a long-term basis while preserving SRA was 26-35 mg L(-1) (200-260 microM). A low level of expression of the mRNA of the sulfite reductase subunit alpha (dsrA) gene was obtained in the presence of the TCE and its intermediate products. This gene was associated to SRB belonging to the genera Desulfovibrio, Desulfosalsimonas, Desulfotomaculum, Desulfococcus, Desulfatiglans and Desulfomonas" |
| Keywords: | "Adaptation, Physiological Biodegradation, Environmental Bioreactors/*microbiology Fatty Acids, Volatile/metabolism Feasibility Studies Genes, Bacterial *Sewage/chemistry/microbiology Sulfates/metabolism Sulfur-Reducing Bacteria/*drug effects/genetics Time;" |
| Notes: | "MedlineSantana-Santos, Mario Alberto Ordaz, Alberto Jan-Roblero, Janet Bastida Gonzalez, Fernando Zarate Segura, Paola B Guerrero-Barajas, Claudia eng England 2019/01/25 J Environ Sci Health A Tox Hazard Subst Environ Eng. 2019; 54(5):461-471. doi: 10.1080/10934529.2019.1567157. Epub 2019 Jan 24" |
