« Previous AbstractRegulator of G protein signaling 8 (RGS8) requires its NH2 terminus for subcellular localization and acute desensitization of G protein-gated K+ channels    Next AbstractA sexually conditioned switch of chemosensory behavior in C. elegans »

Biosci Biotechnol Biochem

Title:		"Production of 2-phenylethanol in roses as the dominant floral scent compound from L-phenylalanine by two key enzymes, a PLP-dependent decarboxylase and a phenylacetaldehyde reductase"
Author(s):		Sakai M; Hirata H; Sayama H; Sekiguchi K; Itano H; Asai T; Dohra H; Hara M; Watanabe N;
Address:		"The United Graduate School of Agricultural Science, Gifu University (Shizuoka University), Japan"
Journal Title:		Biosci Biotechnol Biochem
Year:		2007
Volume:		20071007
Issue:		10
Page Number:		2408 - 2419
DOI:		10.1271/bbb.70090
ISSN/ISBN:		0916-8451 (Print) 0916-8451 (Linking)
Abstract:		"We investigated the biosynthetic pathway for 2-phenylethanol, the dominant floral scent compound in roses, using enzyme assays. L-[(2)H8] Phenylalanine was converted to [(2)H8] phenylacetaldehyde and [(2)H8]-2-phenylethanol by two enzymes derived from the flower petals of R. 'Hoh-Jun,' these being identified as pyridoxal-5'-phosphate-dependent L-aromatic amino acid decarboxylase (AADC) and phenylacetaldehyde reductase (PAR). The activity of rose petal AADC to yield phenylacetaldehyde was nine times higher toward L-phenylalanine than toward its D-isomer, and this conversion was not inhibited by iproniazid, a specific inhibitor of monoamine oxidase. Under aerobic conditions, rose petal AADC stoichiometrically produced NH3 together with phenylacetaldehyde during the course of decarboxylation and oxidation, followed by the hydrolysis of L-phenylalanine. Phenylacetaldehyde was subsequently converted to 2-phenylethanol by the action of PAR. PAR showed specificity toward several volatile aldehydes"
Keywords:		"Acetaldehyde/analogs & derivatives/metabolism Alcohol Oxidoreductases/chemistry/*metabolism Aromatic-L-Amino-Acid Decarboxylases/chemistry/*metabolism Kinetics Models, Chemical Molecular Structure Phenylalanine/chemistry/*metabolism Phenylethyl Alcohol/ch;"
Notes:		"MedlineSakai, Miwa Hirata, Hiroshi Sayama, Hironori Sekiguchi, Kazuya Itano, Hiroaki Asai, Tatsuo Dohra, Hideo Hara, Masakazu Watanabe, Naoharu eng Research Support, Non-U.S. Gov't England 2007/10/12 Biosci Biotechnol Biochem. 2007 Oct; 71(10):2408-19. doi: 10.1271/bbb.70090. Epub 2007 Oct 7"