| Title: | Functional analysis of the odorant receptor coreceptor in odor detection in Grapholita molesta (lepidoptera: Tortricidae) |
| Author(s): | Chen XL; Li BL; Chen YX; Li GW; Wu JX; |
| Address: | "Shaanxi Province Key Laboratory of Jujube, College of Life Science, Yan'an University, Yan'an, Shaanxi, China. Key Laboratory of Plant Protection Resources & Pest Management of the Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, China" |
| Journal Title: | Arch Insect Biochem Physiol |
| ISSN/ISBN: | 1520-6327 (Electronic) 0739-4462 (Linking) |
| Abstract: | "The olfactory system must detect and discriminate various semiochemicals in the environment. In response to such diversity, insects have evolved a family of odorant-gated ion channels composed of a common receptor (coreceptor, Orco) and a ligand-binding tuning odorant receptor (OR) that confers odour specificity. This study aims to examine the expression pattern of Orco gene of Grapholita molesta (GmolOrco) and to elucidate the role of GmolOrco in detecting G. molesta sex pheromone and green leaf volatiles by using gene silencing via RNA interference (RNAi) coupled antennal electrophysiological (EAG). Multiple sequence alignment showed that GmolOrco shared high sequence similarities with the Orco ortholog of lepidopterans. The results of real-time quantitative PCR detection demonstrated that GmolOrco was predominantly expressed in adult antennae and had the highest expression quantity in adult period among the different developmental stages. Compared with the noninjected controls, GmolOrco expression in GmolOrcodouble-stranded RNA (dsRNA)-injected males was reduced to 39.92% and that in females was reduced to 40.43%. EAG assays showed that the responses of GmolOrco-dsRNA injected males to sex pheromones (Z)-8-dodecenyl acetate (Z8-12:OAc) and (Z)-8-dodecenyl alcohol (Z8-12:OH) were significantly reduced, and the GmolOrco-dsRNA-injected female to green leaf volatile (Z)-3-hexenyl acetate also significantly declined. We inferred that Orco-mediated olfaction was different in male and female G. molesta adults and was mainly involved in recognizing the sex pheromones released by female moths" |
| Keywords: | "Animals Arthropod Antennae/metabolism Female Gene Expression Genes, Insect Insect Proteins/genetics/metabolism Male *Moths/genetics/metabolism/physiology Odorants Plants/metabolism RNA Interference *Receptors, Odorant/genetics/metabolism Sex Attractants/m;" |
| Notes: | "MedlineChen, Xiu-Lin Li, Bo-Liao Chen, Yu-Xin Li, Guang-Wei Wu, Jun-Xiang eng YDQ2018-22/Research Project of Yan'an University/ 31860506/National Natural Science Foundation of China/ 2018JM3044/Natural Science Basic Research Program of Shaanxi Province, China/ 2019ZDQY-002/Science and Technology Planning Subject of Yan'an City, China/ 2021/07/23 Arch Insect Biochem Physiol. 2021 Oct; 108(2):e21837. doi: 10.1002/arch.21837. Epub 2021 Jul 22" |
