Title: | Purification of sex pheromones specific for pMB1 and pMB2 plasmids of Enterococcus faecalis S-48 |
Author(s): | Quirantes R; Galvez A; Valdivia E; Martin I; Martinez-Bueno M; Mendez E; Maqueda M; |
Address: | "Department of Microbiology, Faculty of Sciences, University of Granada, Spain" |
ISSN/ISBN: | 0008-4166 (Print) 0008-4166 (Linking) |
Abstract: | "The strain Enterococcus faecalis S-48 carries two large conjugative plasmids (pMB1 and pMB2) encoding for antagonistic substances. The pheromone response of these two plasmids was established by purifying the corresponding sex pheromones, using conventional reversed-phase columns. Plasmid pMB1 responds to pheromone cCF10. Plasmid pMB2 responds to a pheromone with an amino acid sequence identical to that of cPD1 (Phe-Leu-Val-Met-Phe-Leu-Ser-Gly). The two pheromone-responding plasmids coexist in a stable fashion in the wild-type strain E. faecalis S-48" |
Keywords: | "Amino Acid Sequence Bacterial Adhesion/drug effects Conjugation, Genetic/*drug effects/genetics Enterococcus faecalis/chemistry/*genetics Molecular Sequence Data Plasmids/*physiology Sequence Analysis Sex Attractants/*isolation & purification/pharmacology;" |
Notes: | "MedlineQuirantes, R Galvez, A Valdivia, E Martin, I Martinez-Bueno, M Mendez, E Maqueda, M eng Research Support, Non-U.S. Gov't Canada 1995/07/01 Can J Microbiol. 1995 Jul; 41(7):629-32. doi: 10.1139/m95-084" |