« Previous AbstractPancreatic ductal adenocarcinoma can be detected by analysis of volatile organic compounds (VOCs) in alveolar air    Next AbstractThe utilisation of nitrogenous compounds by commercial non-Saccharomyces yeasts associated with wine »

Genetics

Title:		Protein-protein interactions in the yeast pheromone response pathway: Ste5p interacts with all members of the MAP kinase cascade
Author(s):		Printen JA; Sprague GF;
Address:		"Department of Chemistry, University of Oregon, Eugene 97403-1229"
Journal Title:		Genetics
Year:		1994
Volume:		138
Issue:		3
Page Number:		609 - 619
DOI:		10.1093/genetics/138.3.609
ISSN/ISBN:		0016-6731 (Print) 0016-6731 (Linking)
Abstract:		"We have used the two-hybrid system of Fields and Song to identify protein-protein interactions that occur in the pheromone response pathway of the yeast Saccharomyces cerevisiae. Pathway components Ste4p, Ste5p, Ste7p, Ste11p, Ste12p, Ste20p, Fus3p and Kss1p were tested in all pairwise combinations. All of the interactions we detected involved at least one member of the MAP kinase cascade that is a central element of the response pathway. Ste5p, a protein of unknown biochemical function, interacted with protein kinases that operate at each step of the MAP kinase cascade, specifically with Ste11p (an MEKK), Ste7p (an MEK), and Fus3p (a MAP kinase). This finding suggests that one role of Ste5p is to serve as a scaffold to facilitate interactions among members of the kinase cascade. In this role as facilitator, Ste5p may make both signal propagation and signal attenuation more efficient. Ste5p may also help minimize cross-talk with other MAP kinase cascades and thus ensure the integrity of the pheromone response pathway. We also found that both Ste11p and Ste7p interact with Fus3p and Kss1p. Finally, we detected an interaction between one of the MAP kinases, Kss1p, and a presumptive target, the transcription factor Ste12p. We failed to detect interactions of Ste4p or Ste20p with any other component of the response pathway"
Keywords:		"*Adaptor Proteins, Signal Transducing Base Sequence Calcium-Calmodulin-Dependent Protein Kinases/genetics/metabolism *Carrier Proteins DNA Primers DNA, Fungal Fungal Proteins/genetics/*metabolism MAP Kinase Kinase Kinases/metabolism Molecular Sequence Dat;"
Notes:		"MedlinePrinten, J A Sprague, G F Jr eng GM 07759/GM/NIGMS NIH HHS/ GM 30027/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1994/11/01 Genetics. 1994 Nov; 138(3):609-19. doi: 10.1093/genetics/138.3.609"