| Title: | Molecular Characterization and Expression Patterns of Two Pheromone-Binding Proteins from the Diurnal Moth Phauda flammans (Walker) (Lepidoptera: Zygaenoidea: Phaudidae) |
| Author(s): | Chen L; Tian Z; Hu J; Wang XY; Wang MQ; Lu W; Wang XP; Zheng XL; |
| Address: | "Guangxi Key Laboratory of Agric-Environment and Agric-Products Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning 530004, China. Xianning Academy of Agricultural Sciences, Xianning 437000, China. Hubei Key Laboratory of Insect Resources Utilization and Sustainable Pest Management, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "Sex pheromone-binding proteins (PBPs) play an important role in sex pheromone recognition in Lepidoptera. However, the mechanisms of chemical communication mediating the response to sex pheromones remain unclear in the diurnal moths of the superfamily Zygaenoidea. In this study, Phauda flammans (Walker) (Lepidoptera: Zygaenoidea: Phaudidae) was used as a model insect to explore the molecular mechanism of sex pheromone perception in the superfamily Zygaenoidea. Two novel pheromone-binding proteins (PflaPBP1 and PflaPBP2) from P. flammans were identified. The two pheromone-binding proteins were predominantly expressed in the antennae of P. flammans male and female moths, in which PflaPBP1 had stronger binding affinity to the female sex pheromones Z-9-hexadecenal and (Z, Z, Z)-9, 12, 15-octadecatrienal, PflaPBP2 had stronger binding affinity only for (Z, Z, Z)-9, 12, 15-octadecatrienal, and no apparent binding affinity to Z-9-hexadecenal. The molecular docking results indicated that Ile 170 and Leu 169 are predicted to be important in the binding of the sex pheromone to PflaPBP1 and PflaPBP2. We concluded that PflaPBP1 and PflaPBP2 may be responsible for the recognition of two sex pheromone components and may function differently in female and male P. flammans. These results provide a foundation for the development of pest control by exploring sex pheromone blocking agents and the application of sex pheromones and their analogs for insect pests in the superfamily Zygaenoidea" |
| Keywords: | Animals Female Male *Moths/physiology *Lepidoptera Pheromones/metabolism *Sex Attractants/chemistry Carrier Proteins/genetics/metabolism Molecular Docking Simulation Insect Proteins/metabolism PflaPBP1 PflaPBP2 diurnal moth pheromone-binding proteins sex; |
| Notes: | "MedlineChen, Lian Tian, Zhong Hu, Jin Wang, Xiao-Yun Wang, Man-Qun Lu, Wen Wang, Xiao-Ping Zheng, Xia-Lin eng 31660206/National Natural Science Foundation of China/ Switzerland 2023/01/09 Int J Mol Sci. 2022 Dec 26; 24(1):385. doi: 10.3390/ijms24010385" |
