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J Biol Chem

Title:		"Three hydroxyproline-rich glycopeptides derived from a single petunia polyprotein precursor activate defensin I, a pathogen defense response gene"
Author(s):		Pearce G; Siems WF; Bhattacharya R; Chen YC; Ryan CA;
Address:		"Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340, USA"
Journal Title:		J Biol Chem
Year:		2007
Volume:		20070420
Issue:		24
Page Number:		17777 - 17784
DOI:		10.1074/jbc.M701543200
ISSN/ISBN:		0021-9258 (Print) 0021-9258 (Linking)
Abstract:		"Hydroxyproline-rich glycopeptides (HypSys peptides) are recently discovered 16-20-amino acid defense signals in tobacco and tomato leaves that are derived from cell wall-associated precursors. The peptides are powerful wound signals that activate the expression of defensive genes in tobacco and tomato leaves in response to herbivore attacks. We have isolated a cDNA from petunia (Petunia hybrida) leaves encoding a putative protein of 214 amino acids that is a homolog of tobacco and tomato HypSys peptide precursors and is inducible by wounding and MeJA. The deduced protein contains a leader sequence and four predicted proline-rich peptides of 18-21 amino acids. Three of the four peptides were isolated from leaves, and each peptide contained hydroxylated prolines and glycosyl residues. Each of the peptides has a -GR- motif at its N terminus, indicating that it may be the substrate site for a processing enzyme. The peptides were active in a petunia suspension culture bioassay at nanomolar concentrations, but they did not induce the expression of defense genes that are directed against herbivores, as found in tobacco and tomato leaves. They did, however, activate expression of defensin 1, a gene associated with inducible defense responses against pathogens"
Keywords:		Amino Acid Sequence Base Sequence Defensins/genetics/*metabolism Glycopeptides/genetics/*metabolism Hydroxyproline/*metabolism Molecular Sequence Data Petunia/genetics/*metabolism Plant Proteins/genetics/*metabolism Protein Precursors/genetics/*metabolism;
Notes:		"MedlinePearce, Gregory Siems, William F Bhattacharya, Ramcharan Chen, Yu-Chi Ryan, Clarence A eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2007/04/24 J Biol Chem. 2007 Jun 15; 282(24):17777-84. doi: 10.1074/jbc.M701543200. Epub 2007 Apr 20"