« Previous AbstractSteroid profiling in the study of rat testicular steroidogenesis    Next Abstract"A resting box for outdoor sampling of adult Anopheles arabiensis in rice irrigation schemes of lower Moshi, northern Tanzania" »

Curr Genet

Title:		REO1 and ROX1 are alleles of the same gene which encodes a transcriptional repressor of hypoxic genes in Saccharomyces cerevisiae
Author(s):		Kwast KE; Burke PV; Brown K; Poyton RO;
Address:		"Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309-0347, USA"
Journal Title:		Curr Genet
Year:		1997
Volume:		32
Issue:		6
Page Number:		377 - 383
DOI:		10.1007/s002940050291
ISSN/ISBN:		0172-8083 (Print) 0172-8083 (Linking)
Abstract:		"Both the REO1 and ROX1 genes are thought to encode heme-dependent, transcriptional repressors of hypoxic genes in Saccharomyces cerevisiae. However, genetic complementation studies have yielded conflicting results about whether these are the same or different genes. Because of the central importance of these repressors, which control the expression of nearly all known hypoxic genes in yeast, we have sought to resolve this confusion by comparing the phenotypes of reo1 and rox1 mutants using Northern-blot analyses, performing additional complementation studies, and sequencing the ROX1 gene in reo1 strains. Northern-blot analyses of a reo1 strain show wild-type expression of the aerobic genes examined, but de-repression of the Rox1-regulated, hypoxic genes. Aerobic transcript levels of these hypoxic genes were also de-repressed in a diploid strain created by mating a rox1 disrupted strain with a reo1 strain, indicating that genetic complementation did not occur between these two strains. Sequence analyses of ROX1 in reo1 strains reveal a frame-shift mutation in the 5'-end of its coding region, resulting in a nonsense codon in the sixth position. Taken together, these results provide compelling evidence that reo1 is an allele of ROX1"
Keywords:		"Aerobiosis *Alleles DNA-Binding Proteins/*genetics Gene Expression Regulation, Fungal/genetics Genes, Fungal/*genetics Genetic Complementation Test Mutation Phenotype RNA, Fungal/analysis RNA, Messenger/analysis Repressor Proteins/*genetics Saccharomyces;"
Notes:		"MedlineKwast, K E Burke, P V Brown, K Poyton, R O eng GM30228/GM/NIGMS NIH HHS/ GM39324/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1998/01/31 Curr Genet. 1997 Dec; 32(6):377-83. doi: 10.1007/s002940050291"