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Plant Physiol

Title:		PECTIN ACETYLESTERASE9 Affects the Transcriptome and Metabolome and Delays Aphid Feeding
Author(s):		Kloth KJ; Abreu IN; Delhomme N; Petrik I; Villard C; Strom C; Amini F; Novak O; Moritz T; Albrectsen BR;
Address:		"Department of Plant Physiology, Umea Plant Science Centre, Umea University, S-90187 Umea, Sweden karen.kloth@wur.nl. Laboratory of Entomology, Wageningen University and Research, 6700 AA Wageningen, The Netherlands. Department of Forest Genetics and Physiology, Umea Plant Science Centre, Swedish Agriculture University, S-90183 Umea, Sweden. Laboratory of Growth Regulators, Centre of the Region Hana for Biotechnological and Agricultural Research, Institute of Experimental Botany CAS and Faculty of Science of Palacky University, Slechtitelu 27, CZ-78371 Olomouc, Czech Republic. Department of Chemical Biology and Genetics, Centre of the Region Hana for Biotechnological and Agricultural Research, Faculty of Science, Palacky University, Slechtitelu 27, CZ-78371 Olomouc, Czech Republic. Unite de recherche Inserm 1121, Universite de Lorraine-INRA Laboratoire Agronomie et Environnement ENSAIA, 2 Avenue Foret de Haye, 54518 Vandoeuvre-les-Nancy, France. Department of Plant Physiology, Umea Plant Science Centre, Umea University, S-90187 Umea, Sweden. Department of Biology, Faculty of Science, Arak University, Arak 38156-8-8349, Iran"
Journal Title:		Plant Physiol
Year:		2019
Volume:		20190924
Issue:		4
Page Number:		1704 - 1720
DOI:		10.1104/pp.19.00635
ISSN/ISBN:		1532-2548 (Electronic) 0032-0889 (Print) 0032-0889 (Linking)
Abstract:		"The plant cell wall plays an important role in damage-associated molecular pattern-induced resistance to pathogens and herbivorous insects. Our current understanding of cell wall-mediated resistance is largely based on the degree of pectin methylesterification. However, little is known about the role of pectin acetylesterification in plant immunity. This study describes how one pectin-modifying enzyme, PECTIN ACETYLESTERASE 9 (PAE9), affects the Arabidopsis (Arabidopsis thaliana) transcriptome, secondary metabolome, and aphid performance. Electro-penetration graphs showed that Myzus persicae aphids established phloem feeding earlier on pae9 mutants. Whole-genome transcriptome analysis revealed a set of 56 differentially expressed genes (DEGs) between uninfested pae9-2 mutants and wild-type plants. The majority of the DEGs were enriched for biotic stress responses and down-regulated in the pae9-2 mutant, including PAD3 and IGMT2, involved in camalexin and indole glucosinolate biosynthesis, respectively. Relative quantification of more than 100 secondary metabolites revealed decreased levels of several compounds, including camalexin and oxylipins, in two independent pae9 mutants. In addition, absolute quantification of phytohormones showed that jasmonic acid (JA), jasmonoyl-Ile, salicylic acid, abscisic acid, and indole-3-acetic acid were compromised due to PAE9 loss of function. After aphid infestation, however, pae9 mutants increased their levels of camalexin, glucosinolates, and JA, and no long-term effects were observed on aphid fitness. Overall, these data show that PAE9 is required for constitutive up-regulation of defense-related compounds, but that it is not required for aphid-induced defenses. The signatures of phenolic antioxidants, phytoprostanes, and oxidative stress-related transcripts indicate that the processes underlying PAE9 activity involve oxidation-reduction reactions"
Keywords:		"Acetylesterase/*metabolism Animals Aphids/*physiology Arabidopsis/*genetics/*metabolism/parasitology Arabidopsis Proteins/*metabolism Down-Regulation/genetics Gene Expression Regulation, Plant Gene Regulatory Networks Genes, Regulator Glucosinolates/metab;"
Notes:		"MedlineKloth, Karen J Abreu, Ilka N Delhomme, Nicolas Petrik, Ivan Villard, Cloe Strom, Cecilia Amini, Fariba Novak, Ondrej Moritz, Thomas Albrectsen, Benedicte R eng Research Support, Non-U.S. Gov't 2019/09/26 Plant Physiol. 2019 Dec; 181(4):1704-1720. doi: 10.1104/pp.19.00635. Epub 2019 Sep 24"